文献类型：期刊文献

中文题名：复方苁蓉颗粒总黄酮提取工艺优化及其体外抗氧化活性

英文题名：Extraction optimization for total flavonoids from Compound Congrong Granules and the in vitro antioxidant activity

作者：高建德[1];宋开蓉[1];朱晓玉[1];刘雄[1];陈晖[1]

第一作者：高建德

机构：[1]甘肃中医药大学,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2018

卷号：40

期号：7

起止页码：1496

中文期刊名：中成药

外文期刊名：Chinese Traditional Patent Medicine

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2017_2018】；

基金：甘肃省中医药管理局科研项目(GZK-2015-27);甘肃省中医方药挖掘与创新转化重点实验室开放基金项目(ZYFYZH-KJ-2015-002);甘肃省科技计划项目(1308RJZA207)

语种：中文

中文关键词：复方苁蓉颗粒;总黄酮;提取;体外抗氧化活性;响应面法

外文关键词：Compound Congrong Granules;total flavonoids;extraction;in vitro antioxidant activity;response surface method

摘要：目的优化复方苁蓉颗粒总黄酮提取工艺,并评价其体外抗氧化活性。方法在单因素试验基础上,以乙醇体积分数、料液比、提取时间为影响因素,总黄酮提取率为评价指标,响应面法优化提取工艺。然后,测定总黄酮对DPPH·、·OH自由基的清除作用。结果最佳条件为乙醇体积分数65%,料液比1∶10,提取时间2.5 h,总黄酮提取率20.93%。总黄酮对2种自由基有明显的清除作用,并呈量效关系。结论该方法稳定可靠,可用于提取具有显著体外抗氧化活性的复方苁蓉颗粒总黄酮。
AIM To optimize the extraction for total flavonoids from Compound Congrong Granules and to evaluate the in vitro antioxidant activity. METHODS With ethanol concentration,solid-liquid ratio and extraction time as influencing factors,extraction rate of total flavonoids as an evaluation index,response surface method was applied to optimizing the extraction on the basis of single factor test. And the scavenging effects of total flavonoids on DPPH·,·OH free radicals were determined. RESULTS The optimal conditions were determined to be 65%for ethanol concentration,1 ∶ 10 for solid-liquid ratio,and 2. 5 h for extraction time,total flavonoids demonstrated the extraction rate of 20. 93%. The total flavonoids exhibited obvious scavenging effects on two free radicals in a dose-dependent manner. CONCLUSION This stable and reliable method can be used for the extraction for total flavonoids with significant in vitro antioxidant activity from Compound Congrong Granules.