文献类型：期刊文献

英文题名：Severe acute respiratory syndrome coronavirus 2 virus-like particles induce dendritic cell maturation and modulate T cell immunity

作者：Mi, Youjun[1,2,3];Liang, Lijuan[4];Xu, Kun[5];Li, Qing[3];Wang, Wenting[5];Dang, Wenrui[1,2];Deng, Jiahui[3];Zhi, Yucheng[6];Li, Xuefeng[6];Tan, Jiying[5]

第一作者：Mi, Youjun

通信作者：Mi, YJ[1];Mi, YJ[2];Mi, YJ[3];Tan, JY[4]

机构：[1]Lanzhou Univ, Sch Basic Med Sci, Gansu Prov Key Lab Evidence Based Med & Clin Trans, Lanzhou, Peoples R China;[2]Lanzhou Univ, Sch Basic Med Sci, Lanzhou Ctr TB Res, Lanzhou, Peoples R China;[3]Lanzhou Univ, Sch Basic Med Sci, Inst pathophysiol, Lanzhou, Peoples R China;[4]Gansu Univ Chinese Med, Clin Med Coll 1, Lanzhou, Peoples R China;[5]Lanzhou Univ, Sch Basic Med Sci, Inst Immunol, Lanzhou, Peoples R China;[6]Lanzhou Univ, Sch Basic Med Sci, Inst Combined Western & Chinese Tradit Med, Lanzhou, Peoples R China

第一机构：Lanzhou Univ, Sch Basic Med Sci, Gansu Prov Key Lab Evidence Based Med & Clin Trans, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Basic Med Sci, Gansu Prov Key Lab Evidence Based Med & Clin Trans, Lanzhou, Peoples R China;[2]corresponding author), Lanzhou Univ, Sch Basic Med Sci, Lanzhou Ctr TB Res, Lanzhou, Peoples R China;[3]corresponding author), Lanzhou Univ, Sch Basic Med Sci, Inst pathophysiol, Lanzhou, Peoples R China;[4]corresponding author), Lanzhou Univ, Sch Basic Med Sci, Inst Immunol, Lanzhou, Peoples R China.

年份：2022

卷号：12

外文期刊名：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85142449644);WOS:【SCI-EXPANDED(收录号:WOS:000887089400001)】；

基金：Funding This research was funded by the Gansu Science and Technology Project (21JR7RA443), Science and Technology Innovation Project of Gansu Provincial Department of Education (2022B-118), Main Research and Development Program of Gansu Province in 2020 (20YF8FA072), Lanzhou Science and Technology Planning Project (2020-XG-33), Key Laboratory of Dunhuang Medical and Translational Education Ministry Project (DHXY20-08).

语种：英文

外文关键词：SARS-CoV-2; COVID-19; virus-like particles (VLPs); dendritic cells; T cell

摘要：Dendritic cells (DCs) are professional antigen-presenting cells that play an important role in both innate and acquired immune responses against pathogens. However, the role of DCs in coronavirus disease 2019 (COVID-19) is unclear. Virus-like particles (VLPs) that structurally mimic the original virus are one of the candidates COVID-19 vaccines. In the present study, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) VLPs were used as an alternative to live virus to evaluate the interaction of the virus with DCs. The results revealed that SARS-CoV-2 VLPs induced DC maturation by augmenting cell surface molecule expression (CD80, CD86, and major histocompatibility complex class II (MHC-II)) and inflammatory cytokine production (tumor necrosis factor-alpha, interleukin (IL)-1 beta, IL-6, and IL-12p70) in DCs via the mitogen-activated protein kinase and nuclear factor-kappa B signaling pathways. In addition, mature DCs induced by SARS-CoV-2 VLPs promoted T cell proliferation, which was dependent on VLPs concentration. Our results suggest that SARS-CoV-2 VLPs regulate the immune response by interacting with DCs. These findings will improve the understanding of SARS-CoV-2 pathogenesis and SARS-CoV-2 vaccine development.