文献类型：期刊文献

英文题名：Applications and challenges of tear proteomics in unveiling the molecular mechanisms and biomarkers of dry eye disease

作者：Hao, Hongyan[1];Li, Chen[1];Zhao, Yu[1];He, Xiafei[1];Dong, Yanping[1];Wang, Yujin[1];Yang, Kan[1]

第一作者：Hao, Hongyan

通信作者：Yang, K[1]

机构：[1]Gansu Univ Chinese Med, Peoples Hosp Lanzhou 1, Clin Med Coll 2, Dept Ophthalmol, 1 Wujiayuan Western St,Dunhuang Rd, Lanzhou 730050, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Peoples Hosp Lanzhou 1, Clin Med Coll 2, Dept Ophthalmol, 1 Wujiayuan Western St,Dunhuang Rd, Lanzhou 730050, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2026

卷号：23

期号：1

外文期刊名：CURRENT PROTEOMICS

收录：;Scopus(收录号：2-s2.0-105032244642);WOS:【SCI-EXPANDED(收录号:WOS:001721255600001)】；

基金：Scientific Research and Technology Development Program of Lanzhou (2023-ZD-131) ;the Natural Science Foundation of Gansu Province (24JRRA770) .

语种：英文

外文关键词：Dry eye disease; Molecular mechanism; Biomarkers; Tear fluid; Proteomics

摘要：Dry Eye Disease (DED) stands as one of the most prevalent ocular surface disorders globally, with an increasing incidence that significantly impairs patients' visual function and quality of life. Conventional diagnostic approaches, which rely on subjective symptom questionnaires and tests of tear film stability, often lack sufficient sensitivity and specificity and fail to accurately delineate underlying etiologies. Tear fluid, a non-invasive biological sample that directly mirrors the ocular surface microenvironment, is rich in proteins, lipids, and metabolites, offering an ideal resource for investigating disease mechanisms and identifying biomarkers. Driven by advances in mass spectrometry (MS) and quantitative proteomics, tear proteomics has emerged as a pivotal tool for elucidating the molecular mechanisms of DED and discovering potential biomarkers. This article provides a systematic review of current methodologies for tear sample processing and proteomic analysis. It focuses on the application of tear proteomics in uncovering key molecular mechanisms involved in DED, including inflammatory responses, oxidative stress, tear film instability, and neurofunctional dysregulation. Furthermore, it summarizes the current landscape of potential biomarker candidates and their validation status. The review also critically examines prevailing challenges in the field, such as sample heterogeneity, technical standardization issues, and barriers to clinical translation. Finally, it outlines promising future directions, aiming to provide a foundation for the precision diagnosis and targeted therapy of DED.