文献类型：期刊文献

中文题名：碱性盐及混合盐碱胁迫对蒙古黄芪种子萌发和幼苗生理特性的影响

英文题名：Effect of basic salt and mixed salt-alkali stress tolerance on seed germination and seedling physiological characteristics of Astraglus membranaceus var. mongholicus

作者：韩多红[1];张勇[1];晋玲[2]

第一作者：韩多红

机构：[1]河西学院农业与生物技术学院,甘肃张掖734000;[2]甘肃中医学院,甘肃兰州730000

第一机构：河西学院农业与生物技术学院,甘肃张掖734000

年份：2013

卷号：44

期号：12

起止页码：1661

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家科技支撑计划项目(2007BAI37B01);国家中医药管理局2012年中医药行业科研专项(201207002);河西学院青年教师科研基金项目(QN2012-13)

语种：中文

中文关键词：蒙古黄芪;碱;混合盐碱;种子萌发;幼苗生理特性

外文关键词：Astraglus membranaceus（Fisch.） Bge.var.mongholicus（Bge.） Hsiao.; alkali; mixed salt-alkali; seed germination; seedling physiological characteristics

摘要：目的比较不同浓度的Na2CO3和NaCl+Na2CO3处理下蒙古黄芪种子萌发和幼苗的部分生理指标,分析2种盐对蒙古黄芪的胁迫程度大小,寻找提高蒙古黄芪种子及幼苗在盐碱胁迫下抗性能力的途径。方法测定不同浓度的Na2CO3和NaCl+Na2CO3胁迫下蒙古黄芪种子的发芽势(Gv)、发芽率(Gr)、相对发芽率、相对盐害率,并对幼苗的叶绿素量、可溶性蛋白质量、丙二醛(MDA)量、超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性进行了测定。结果较低浓度的Na2CO3和NaCl+Na2CO3处理,对种子的萌发具有促进作用,较高浓度的处理对种子萌发有抑制作用,且抑制程度随处理浓度的增加而加强,各处理的各项萌发指标与对照相比,都具有显著的差异(P<0.05)。随着2种处理液浓度的增加,叶绿素量随之逐渐减少,可溶性蛋白质的量也逐渐降低,二者都与处理浓度呈负相关;但幼苗中MDA量呈增加趋势,与处理浓度呈正相关。随着2种处理浓度的增加,SOD、POD活性均不同程度地表现为先上升后下降的趋势,在50 mmol/L时,达到了最大值。结论 NaCl+Na2CO3对蒙古黄芪种子及幼苗的胁迫程度大于Na2CO3。
Objective To compare the effects of Na2CO3 and NaCl＋ Na2CO3 at different concentration on the seed germination and seedling physiological characteristics of Astraglus membranaceus var.mongholicus,analyze the stress degrees of the both two on A.membranaceus var.mongholicus,and search for the effective ways for improving the resistance of seeds and seedlings under salt stress.Methods Several physiological indexes of A.membranaceus var.mongholicus seeds under different salt treatments,such as the germination vigor（Gv）,germination rate（Gr）,relative germination rate,and relative salt damage rate were measured.And other indexes of the seedlings like chlorophyll contents,soluble protein contents,the contents of malondialdehyde（MDA）,the activities of superoxide dismutase（SOD）,and peroxidase（POD） were also measured.Results Na2CO3 and NaCl ＋ Na2CO3 at lower concentration could promote the seed germination but inhibit the seed germination at higher concentration.The inhibition was increased with the concentration increasing.The germination indexes of each treatment had the significant differences compared with the control（P〈 0.05）.With the salt concentration increasing,the contents of chlorophyll and the soluble protein decreased,which was negatively correlated with the concentration.The content of MDA was increased and positively correlated with the concentration.The change trend of SOD activity showed the first increase and then decreased with the salt concentration increasing,which was similar to that of POD.The activities of both SOD and POD reached the peak at 50 mmol/L.Conclusion The stress of NaCl＋Na2CO3 is higher than that of Na2CO3 on the seeds and seedlings of A.membranaceus var.mongholicus.