文献类型：期刊文献

中文题名：黄芩变异种质的生根壮苗研究

英文题名：Study on Rapid Propagation of Scutellaria baicalensis Variation

作者：张延红[1];高素芳[1];陈红刚[1];李晓霞[1];晋玲[1]

第一作者：张延红

机构：[1]甘肃中医学院药学系

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2013

期号：4

起止页码：511

中文期刊名：中药材

外文期刊名：Journal of Chinese Medicinal Materials

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；PubMed;

基金：国家科技攻关西部支撑项目(2007BA137B01)

语种：中文

中文关键词：白花黄芩;短枝;生根;壮苗

外文关键词：White flower Scutellaria baicalensis Georgi ; Stem segments ; Rooting; Healthy seedling

摘要：目的:建立黄芩变异种质——白花黄芩的生根壮苗技术体系,为保存和进一步利用该特殊种质奠定理论与技术基础。方法:以白花黄芩带腋芽的茎段为外植体,在添加不同种类和浓度的植物生长调节剂和附加物的不同基本培养基中进行生根壮苗培养。结果:适宜白花黄芩生根的培养基为1/2 MS(全减半)+IBA 0.02 mg/L+2%蔗糖,根诱导率高达100%;壮苗培养的最适培养基为1/2 MS(全减半)+PP3330.2 mg/L+IBA 0.02 mg/L+2%蔗糖,试管苗绿色,节间正常,生长健壮。结论:1/2 MS(全减半)培养基和较低浓度的蔗糖有利于黄芩试管苗生根;培养基中适当添加IBA能显著提高生根率,根粗壮,根条数多;PP333对白花黄芩试管苗的生长具有明显的矮化作用,适宜浓度能明显改善试管苗的素质,试管苗生长健壮。该试验建立了良好的白花黄芩试管苗的生根壮苗技术。
Objective：To establish and optimize the technology of taking root and promoting seedlings of white flower Scutellaria baicalensis test tube plantlet, and provide the theory and technology base for efficient factorization production system of white flower Scutellaria baicalensis. Methods ： Stem segments with axillary bud were cultured onto the different basic medium with different kinds and concentration of cytokinin and auxins to take root and produce strong seedling. Results：The suitable culture medium for taking root of white flower Scutellaria baicalensis was 1/2 MS（ all substance reduced half） ＋ IBA 0.02 mg/L ＋ sucrose 2% ,the induction rate of root was 100% ;The best medium for promoting seedling was 1/2 MS（ all substance reduced half） ＋ PP3330. 2 mg/L ＋ IBA 0.02 mg/L ＋ sucrose 2% ,the seedling was green,the internode was normal,and its growth was vigorous and healthy. Conclusion： 1/2 MS（all substance reduced half）culture medium and relatively low concentration of sucrose is beneficial to inducing roots;Media adding appropriate concentration of IBA can significantly increase the root induction rate, the seedling has many stout roots;PP333 has dwarfing effect on seedlings and suitable concentration of PP333 can significantly improve the quality of the plantlets. A good technology of taking root and producing strong test tube plantlets is established.