文献类型：期刊文献

中文题名：慢性阻塞性肺疾病大鼠模型病变特点的研究

英文题名：Characteristics of Pathological Changes in Chronic Obstructive Pulmonary Disease (COPD) Rat Model

作者：张莉[1];李金田[1];刘永琦[1];李娟[1];张毅[1]

第一作者：张莉

机构：[1]甘肃中医学院,兰州730000

第一机构：甘肃中医药大学

年份：2010

卷号：29

期号：2

起止页码：285

中文期刊名：四川动物

外文期刊名：Sichuan Journal of Zoology

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：2003甘肃省科计攻关项目(No.2GS035-A43-067);2005甘肃省教育厅导师项目(No.0505-02)

语种：中文

中文关键词：嗅鞘细胞;嗅觉神经成纤维细胞;原代培养;免疫细胞化学慢性阻塞性肺疾病;病变特点;实验研究

外文关键词：COPD ; characteristics of pathological changes ; experimental study

摘要：目的研究慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)大鼠模型肺表面活性物质相关蛋白A(Surfactant protein A,SP-A)表达及肺组织超微结构变化特点。方法运用气管内滴注脂多糖加烟熏28天法建立COPD大鼠模型。观察大鼠一般状态、用免疫组化法检测SP-A表达及透射电镜观察肺泡Ⅱ型上皮细胞超微结构变化。结果各组SP-A平均肺泡阳性细胞数与空白组比较有显著差异(P<0.01)。结论用气管内滴注脂多糖加烟熏28天法建立的COPD大鼠模型可靠,造模停止两周后模型稳定。
Objective To study the expression of pulmonary surface-active substance related protein A （ Surfactant protein A, SP-A） of chronic obstructive pulmonary disease （COPD） rat model and uhrastructural changes in the lung tissue of COPD. Methods The rat model of COPD was established by both intratracheal instillation of lipopolysaccharide （LPS） and passive smoking for 28 days. Observe the general condition of rats, test the expression level of Surfactant protein A （SP- A） by immunohistochemistry, and observe the uhrastructural changes of alveolar type Ⅱ epithelial cells through transmission electron microscope. Results Compared with the blank group, the average numbers of SP-A-positive cells per alveolus in other groups showed significant differences （ P 〈 0.01 ）. Conclusion It is reliable that the rat model of COPD was established by the combination of intratracheal instillation of lipopolysaceharide （LPS） and passive smoking for 28 days, and the model was still stable after ending the combination two weeks later.