文献类型：期刊文献

中文题名：甘肃党参水煎剂对D-半乳糖诱导衰老小鼠免疫功能的影响

英文题名：Effects of Gansu Dangshen decoction on the immune function of D-galactose induced aging mice

作者：何敏[1];伍春[1];明海霞[1];侯茜[1];张帆[1]

第一作者：何敏

机构：[1]甘肃中医学院系统生物学与中医药转化研究所,中西医结合基础学科重点实验室

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）|甘肃中医药大学中西医结合学院

年份：2013

卷号：29

期号：8

起止页码：794

中文期刊名：细胞与分子免疫学杂志

外文期刊名：Chinese Journal of Cellular and Molecular Immunology

收录：MEDLINE(收录号：23948401);CSTPCD;;Scopus(收录号：2-s2.0-84887209622);北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；PubMed;

基金：甘肃省高等学校研究生导师科研项目(1006-01)

语种：中文

中文关键词：党参;D-半乳糖;免疫功能;延缓衰老

外文关键词：Dangshen; D-galactose; immune function; anti-aging

摘要：目的研究甘肃党参水煎剂对D-半乳糖诱导衰老小鼠免疫功能的影响。方法采用D-半乳糖诱导亚急性衰老小鼠模型,测定每组小鼠的体质量,计算脾脏指数和胸腺指数,观察脾脏组织超微结构,采用免疫组化法检测CD138的表达,ELISA检测小鼠血清中IgG、IgM和补体C3、C4含量。结果与正常对照组相比,衰老模型组小鼠的脾脏指数和胸腺指数显著降低(P<0.01),与模型组比较,高、低剂量党参组和维生素E组小鼠的脾脏指数和胸腺指数显著升高(P<0.01)。正常对照组脾小体中淋巴细胞的超微结构均正常,而模型组小鼠的脾脏超微结构有明显的损伤现象。低、高剂量党参组和维生素E组均使已受损伤的脾脏超微结构得以修复,其中高剂量党参组使受损的脾脏超微结构恢复到最接近正常水平。与正常对照组比较,衰老模型组小鼠脾脏组织中CD138的表达率较低(P<0.01),小鼠血清中IgG、IgM和补体C3、C4含量显著减少(P<0.01或P<0.05);与衰老模型组比较,正常对照组和高剂量党参组小鼠脾脏组织中CD138的表达率较高(P<0.01),小鼠血清中IgG、IgM和补体C3、C4含量显著增加(P<0.01或P<0.05),而党参低剂量组和维生素E组无显著差异(P>0.05)。结论甘肃党参水煎剂具有增强D-半乳糖诱导衰老小鼠免疫功能的作用。
Objective To study the effect of Chinese herb Dangshen decoction on the immune function of D-galactose-induced aging mice. Methods A total of 60 mice were randomly divided into 5 groups, namely normal control group, aging model group, high and low concentration Dangshen treated groups, vitamin E control group. Aging mice models were made by D-galactose. We weighed all mice, determined their indexes of the spleen and thymus, observed the ultrastructure of the spleen, and detected the expression of CD138 by immunohistochemistry. The levels of serum IgG, IgM and complement C3 and CA were detected by ELISA. Results Compared with normal control group, the aging mice had lower indexes of the spleen and thymus （ P 〈 0.01 ）. Compared with aging mice, the indexes rose significantly in Dangshen treated groups and vitamin E group （P〈0.01）. Furthermore, the lymphocytes of the spleen were injured obviously in the aging mice, but the ultrastructure of spleen got repaired in Dangshen treated groups and vitamin E group, especially high-concentration Dangsh-en treated group that presented the lymphocytes nearly as normal as the normal control group. In addition, compared with the normal control group, the aging mice showed a lower expression of CD138 in the spleen （ P 〈 0.01 ）, and the levels of IgG, IgM and complement C3 and CA dropped sharply （P 〈0.01 or P 〈 0.05）. However, they were all significantly higher in the high concentration Dangshen group than in the aging mice （ P 〈 0.01 or P 〈 0.05）, and were not statistically different between the low concentration Dangshen group and the vitamJn E group （P 〉 0.05）. Conclusion The decoction of Chinese herb Dangshen in Gansu can enhance the immune function of D-galactoce-induced aging mice.