文献类型：期刊文献

英文题名：Identification of IMPA2 as the hub gene associated with colorectal cancer and liver metastasis by integrated bioinformatics analysis

作者：Wang, Liuli[1,2,3];Liu, Deming[4];Liu, Shuo[1];Liao, Tianyi[1];Jiao, Yajun[4];Jiang, Xianglai[4];Wang, Yongfeng[5];Chen, Yaqiong[6];Ma, Haizhong[7];Cai, Hui[1,2,3]

第一作者：Wang, Liuli

通信作者：Cai, H[1]

机构：[1]Lanzhou Univ, Clin Med Coll 1, 199 Donggang WestRd, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Prov Hosp, Dept Gen Surg, Lanzhou 730000, Peoples R China;[3]Gansu Prov Hosp, Key Lab Mol Diag & Precis Treatment Surg Tumor, Lanzhou 730000, Peoples R China;[4]Ningxia Med Univ, Yinchuan 750004, Ningxia, Peoples R China;[5]Gansu Univ Tradit Chinese Med, Dept Clin Med, Lanzhou 730000, Peoples R China;[6]Gansu Prov Hosp, Med Dept, Lanzhou 730000, Peoples R China;[7]Gansu Prov Hosp, Dept Qual Control, Lanzhou 730000, Peoples R China

第一机构：Lanzhou Univ, Clin Med Coll 1, 199 Donggang WestRd, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Clin Med Coll 1, 199 Donggang WestRd, Lanzhou 730000, Gansu, Peoples R China.

年份：2022

卷号：21

外文期刊名：TRANSLATIONAL ONCOLOGY

收录：;Scopus(收录号：2-s2.0-85130969104);WOS:【SCI-EXPANDED(收录号:WOS:000866367100004)】；

基金：This study was supported by the National Natural Science Foundation of China (Grant Numbers ZYYDDFFZZJ-1), Natural Science Foundation of Gansu Province, China (Grant Numbers 18JR3RA052) and National Key Research and Development Program (grant numbers 2018YFC1311500).

语种：英文

外文关键词：Colorectal cancer; Colorectal liver metastasis; Weighted gene co-expression network analysis; Differentially expressed gene analysis; Integrated bioinformatics analysis

摘要：Background and Objectives: Colorectal cancer (CRC) is one of the most common malignant tumors worldwide with high incidence and mortality rate, while colorectal liver metastasis (CRLM) is one of the major causes of cancer-related deaths. Therefore, the present study aims to identify the hub gene associated with CRC carcinogenesis and liver metastasis, and then explore its diagnostic and prognostic value as well as the potential regulation mechanism. Methods: The overlapping differential co-expression genes among CRC, CRLM, and normal tissues were explored on the GSE49355 and GSE81582 datasets from the Gene Expression Omnibus (GEO) database by integrated bioinformatics analysis. Then, the hub prognostic genes were selected from the overlapping genes by univariate Cox proportional hazard analysis and online database Gene Expression Profiling Interactive Analysis 2 (GEPIA2). Subsequently, the clinical value of the hub genes was evaluated in the TCGA and GSE39582 cohorts. Finally, the underlying mechanisms of the hub gene regulating CRC carcinogenesis and metastasis were explored by Gene function annotation and DNA methylation analysis. Results: Inositol mono-phosphatase 2 (IMPA2) was identified as the hub gene associated with CRC carcinogenesis and liver metastasis. IMPA2 had an excellent diagnostic efficiency, and its expression was significantly decreased in CRC and liver metastasis samples, being positively correlated with poor prognosis. Moreover, its low expression was associated with AJCC stage III+IV, T4, N1+2, and M1. In addition, our results revealed that the potential mechanisms used by IMPA2 to mediate CRC carcinogenesis and metastasis could be associated with lipid metabolism and epithelial mesenchymal transition (EMT). Finally, IMPA2 expression could be regulated by DNA methylation. Conclusions: IMPA2 was identified and reported for the first time as a hub gene biomarker in the diagnosis and prognosis of CRC, which could regulate CRC carcinogenesis and liver metastasis through the regulation of lipid metabolism, EMT, and DNA methylation.