文献类型：期刊文献

英文题名：Knockdown of Trop2 inhibits proliferation and migration and induces apoptosis of endometrial cancer cells via AKT/β-catenin pathway

作者：Sun, Xiaotong[1];Xing, Guangyang[1];Zhang, Cui[2];Lu, Kun[1];Wang, Yuqiong[1];He, Xiyan[1]

第一作者：Sun, Xiaotong

通信作者：Xing, GY[1]

机构：[1]Gansu Prov Peoples Hosp, Dept Gynecol & Obstet, 204 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Dept Pathol, Affiliated Hosp, Lanzhou, Peoples R China

第一机构：Gansu Prov Peoples Hosp, Dept Gynecol & Obstet, 204 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Gansu Prov Peoples Hosp, Dept Gynecol & Obstet, 204 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.

年份：2020

卷号：38

期号：2

起止页码：141

外文期刊名：CELL BIOCHEMISTRY AND FUNCTION

收录：;Scopus(收录号：2-s2.0-85078680498);WOS:【SCI-EXPANDED(收录号:WOS:000508462200001)】；

基金：Natural Science Foundation of Gansu Province, Grant/Award Numbers: 1506RJZ168, 1506RJZ168

语种：英文

外文关键词：AKT; beta-catenin pathway; apoptosis; endometrial cancer; epithelial-to-mesenchymal transition; proliferation; Trop2

摘要：Endometrial cancer (EC) is the most common gynaecologic malignancy in western countries and has been reported to account for about 7% of female malignant tumours and 20% to 30% of female genital system malignant tumours. Accumulating evidence showed the expression of human trophoblast cell surface antigen 2 (Trop2) was abnormal in many cancers; however, the expression and role of Trop2 in EC are not clear. The Trop-2 protein expression was detected by western blot in EC cells. Cell proliferation, apoptosis, and migration were measured by CCK-8, flow cytometry, and Transwell assay, respectively. The epithelial mesenchymal transition (EMT) and AKT/beta-catenin signalling pathway-related proteins in EC cell lines were detected by western blot assay following Trop2 gene silencing. The present study revealed that the Trop2 protein was highly expressed in EC cell lines compared with human endometrial epithelial cells. The Trop2 mRNA and protein were obviously decreased following transfection with Trop2-siRNA sequence in KLE and Ishikawa cells. Meanwhile, Trop2 gene silencing in KLE and Ishikawa cells strongly inhibited cell proliferation and migration and increased cell apoptosis. Investigation into the molecular mechanism indicated that the Trop2 gene silencing suppressed EMT and AKT/beta-catenin signalling pathway activation. Significance of the study These findings suggested that Trop2 silencing inhibited EC cell proliferation and migration and promoted cell apoptosis. The mechanism might be related to the inhibition of the AKT/beta-catenin signalling pathway in EC cells. Therefore, Trop2 may be a potential therapeutic target for the treatment of EC.