文献类型：期刊文献

英文题名：Protective Effect of p-Cymene on Lipopolysaccharide-Induced Acute Lung Injury in Mice

作者：Chen, Lvyi[1];Zhao, Lei[2];Zhang, Chunfeng[3];Lan, Zhou[4]

第一作者：Chen, Lvyi

通信作者：Zhang, CF[1]

机构：[1]South Cent Univ Nationalities, Coll Pharm, Wuhan 430074, Peoples R China;[2]Gansu Coll Tradit Chinese Med, Key Lab Chem & Qual Tradit Chinese Med, Coll Gansu Prov, Lanzhou 730000, Peoples R China;[3]China Pharmaceut Univ, Key Lab Modern Chinese Med, Minist Educ, Nanjing 210009, Peoples R China;[4]Hubei Univ Chinese Med, Sch Pharm, Wuhan 430065, Peoples R China

第一机构：South Cent Univ Nationalities, Coll Pharm, Wuhan 430074, Peoples R China

通信机构：[1]corresponding author), China Pharmaceut Univ, Key Lab Modern Chinese Med, Minist Educ, Nanjing 210009, Peoples R China.

年份：2014

卷号：37

期号：2

起止页码：358

外文期刊名：INFLAMMATION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000332715000008)】；

基金：The project was supported by Chinese National Project of "Twelfth Five-Year" Plan for Science & Technology Support (2012BAI27B06), the Special Fund for Basic Scientific Research of Central Colleges, South-Central University for Nationalities (no. CZY13016), the Natural Science Foundation of China (81303284) and the Planning of Deeply Participating into the Enterprises for Young Teachers (XD2012060).

语种：英文

外文关键词：p-cymene; inflammation; LPS-induced acute lung injury; NF-kappa B

摘要：In the previous study, the anti-inflammatory effect of p-cymene had been found. In this study, we investigated anti-inflammatory effects of p-cymene on acute lung injury using lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. The cell counting in the bronchoalveolar lavage fluid (BALF) was measured. The animal lung edema degree was evaluated by wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) activity and myeloperoxidase (MPO) activity was assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators including tumor necrosis factor alpha (TNF-alpha), IL-1 beta, and IL-6 were assayed by enzyme-linked immunosorbent assay method. The pathological changes of the lung tissues were observed by hematoxylin and eosin staining. The inflammatory signal pathway-related protein levels of NF-kappa B were measured using Western blotting. The data showed that treatment with the p-cymene markedly attenuated inflammatory cell numbers in the BALF, decreased NF-kappa B protein level in the lungs, improved SOD activity, and inhibited MPO activity. Histological studies demonstrated that p-cymene substantially inhibited LPS-induced neutrophils in the lung tissue compared with the model group. The results indicated that p-cymene had a protective effect on LPS-induced ALI in mice.