文献类型：期刊文献

英文题名：Effects of Altitude and Duration of Different Levels of Hypoxic Exposure on Hypoxia-Inducible Factor-1α in Rat Tissues

作者：Li, Xiao-lin[1,3,6];Wang, Wei-gang[1,3];Li, Mao-xing[1];Liu, Tian-long[2,3];Tian, Xiu-yu[2,4];Wu, Lan[5]

第一作者：Li, Xiao-lin

通信作者：Li, XL[1]

机构：[1]Chinese Peoples Liberat Army, Hosp Joint Logist Support Force 940, Dept Clin Pharm, Lanzhou, Peoples R China;[2]Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[3]Gansu Plateau Pharmaceut Technol Ctr, Lanzhou, Peoples R China;[4]Lanzhou Univ, Coll Pharm, Lanzhou, Peoples R China;[5]Northwest Minzu Univ, Inst Chem Technol, Lanzhou, Peoples R China;[6]Chinese Peoples Liberat Army, Hosp Joint Logist Support Force 940, Dept Clin Pharm, 333 South Binhe Rd, Lanzhou 730050, Peoples R China

第一机构：Chinese Peoples Liberat Army, Hosp Joint Logist Support Force 940, Dept Clin Pharm, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Chinese Peoples Liberat Army, Hosp Joint Logist Support Force 940, Dept Clin Pharm, 333 South Binhe Rd, Lanzhou 730050, Peoples R China.

年份：2022

卷号：23

期号：2

起止页码：173

外文期刊名：HIGH ALTITUDE MEDICINE & BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000807377300001)】；

基金：This work was supported by the project of the Fundamental Research Funds for the Central Universities (31920200010), the major research and development pro-gram of Gansu Province (20YF3FA035), the Emergency Medical Research Project of Novel Coronavirus Pneumonia(COVID-19) (20yjky018), and a Youth Development Project(2021yxky060).

语种：英文

外文关键词：HIF-1 alpha; hypoxia altitude; hypoxia duration

摘要：Objective: This research aimed to evaluate the effects of hypoxia at different altitudes and durations on the expression of hypoxia-inducible factor-1 alpha (HIF-1 alpha) in rat tissues. Methods: A total of 72 Wistar rats were used to investigate the effect of hypoxia at different durations on rat tissues and 72 Wistar rats were used to investigate the effect of hypoxia at different altitudes. Hematoxylin and Eosin (HE) staining was performed to observe the pathological changes of hippocampus tissues, and the expression of HIF-1 alpha of rats under conditions of hypoxia was detected by quantitative real-time polymerase chain reaction and western blotting. Results: According to the pathological results, we found that the degree of the brain, lung, and heart damage and HIF-1 alpha expression, showed an increasing trend as the altitude (1,500, 3,000, 4,500, 6,000, 7,500, and 8,000 m for 12 hours) and duration (0, 6, 12, 24, 36, and 72 hours at 7,500 m) of hypoxia increased. Although there is a significant difference at 8,000 m, considering model stability, animal ethics and cost, we chose 7,500 m as a fixed altitude during hypoxia at different durations. Compared with the normoxic group, the expression of HIF-1 alpha mRNA in the 7,500 m significantly increased by 30.48%, 21.00%, and 12.62%, in brain, lung, and heart tissue (p < 0.01), and HIF-1 alpha mRNA in the 72-hour hypoxic exposure group significantly increased by 52.58%, 20.39%, 27.88% in tissues (p < 0.05). Compared with the normoxic group, HIF-1 alpha protein expressions in the 7,500 m significantly increased by 10.26%, 31.71%, and 13.33% in brain, lung, and heart tissue (p < 0.01, p < 0.01, p < 0.05), and HIF-1 alpha protein expressions in the 72-hour hypoxic exposure group significantly increased by 18.89%, 22.89%, and 29.75% in tissues (p < 0.05). Conclusion: HIF-1 alpha expression in the rat was correlated with altitude and duration of hypoxic exposure.