文献类型：期刊文献

中文题名：黄芪对高血压大鼠血管重构中内质网应激反应的影响

英文题名：Effect of Astragalus on endoplasmic reticulum stress during the vascular remodeling in hypertensive rats

作者：顾静[1];郭超[1];车敏[1];吴红彦[1];李海龙[1]

第一作者：顾静

机构：[1]甘肃中医药大学

第一机构：甘肃中医药大学

年份：2019

卷号：27

期号：1

起止页码：65

中文期刊名：中国实验动物学报

外文期刊名：Acta Laboratorium Animalis Scientia Sinica

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：甘肃省中医药管理局科研课题(GZK-2014-79);敦煌医学与转化省部共建教育部重点实验室开放基金项目(DHYX14-004);甘肃省青年科技基金计划(1506RJYA037)~~

语种：中文

中文关键词：内质网应激;黄芪;高血压;血管重构.

外文关键词：endoplasmic reticulum stress;Astragalus;hypertension;vascular remodeling;aorta;rat

摘要：目的研究黄芪对高血压大鼠血管重构中内质网应激反应(ERS)的影响,并探讨其血管保护的分子机制。方法将140只大鼠分为对照组、模型组、干预组。采用腹主动脉狭窄术建立高血压大鼠模型,干预组大鼠腹腔注射黄芪注射液8 g/(kg·d)。各组术后1、2、4、6周时采用鼠尾动脉测压法测量大鼠血压,测量血管肌层厚度、Western blot检测CRT和caspase-12的表达、TUNEL法检测血管平滑肌细胞(VSMC)凋亡率。结果模型组术后VSMC形态改变,血压、动脉血管壁肌层厚度和VSMC凋亡率可时间依赖性增大,ERS分子CRT在术后1、2周表达显著升高,4、6周表达降低,而caspase-12分子2周以后表达才升高,且随时间推迟,这种高表达越显著。黄芪干预对比模型组,VSMC形态有一定改善,血压、血管壁肌层厚度和VSMC凋亡率均显著降低,6周时降低幅度最大,同时黄芪能抑制CRT的早期高表达,能抑制caspase-12的高表达,这种抑制作用随着时间的推迟越明显。结论黄芪对高血压大鼠有一定降压作用,可改善血管重构,其机制可能与其调节ERS保护性和促凋亡因子有关。
Objective To observe the effect of Astragalus injection on endoplasmic reticulum stress during the vascular remodeling in hypertensive rats, and investigate its molecular mechanism of vascular protection. MethodsMale Wistar rats were randomly divided into control group, model group and intervention group.“Abdominal aortic stenosis” methods was used to prepare the hypertensive rat model. From the second day after surgery, the rats in the intervention group were injected with Astragalus injection solution 8 g/(kg ·d)intraperitoneally. Blood pressure was measured by rat caudal artery manometry at 1, 2, 4 and 6 weeks after the operation. Then, the animals were anesthetized and the aorta was separated, and pathology and immunohistochemistry were performed to determine the thickness of vascular muscle layer using an image analysis system. The expression of CRT and caspase-12 was detected by Western blotting, and the apoptosis rate of VSMC was determined by TUNEL assay. ResultsIn the model group, pathological changes of VSMC were observed, and blood pressure, the thickness of the artery wall muscle layer and the apoptosis rate of VSMC were increased in a time-dependent manner. CRT expression was significantly increased at 1-2 weeks after the operation, but decreased at 4-6 weeks. Caspase-12 expression was increased 2 weeks after the surgery, and the increase in expression became more significant over time. In the Astragalus intervention group, the morphology of VSMC was improved to some extent. Blood pressure, the thickness of vascular wall muscle layer and the apoptosis rate of VSMC were significantly decreased. The biggest decrease was observed at 6 weeks after Astragalus intervention. Astragalus inhibited the early high expression of CRT and the high expression of caspase-12, and this inhibitory effect became more obvious as time progressed. Conclusions Astragalus decreases hypertension in rats to some degree and improves aortic vascular remodeling. Its mechanism may be related to the regulation ofERS molecules.