文献类型：期刊文献

中文题名：基于气相色谱-质谱联用技术的道地与近道地产区当归挥发油化学组分分析

英文题名：Analysis of chemical constituents of volatile oil from Angelica sinensis of authentic and near authentic producing areas by GC-MS

作者：李钦[1];王引权[1,2];张慧[1];荔淑楠[1];雒军[1];彭桐[1]

第一作者：李钦

机构：[1]甘肃中医药大学药学院,甘肃兰州730101;[2]甘肃省中药质量与标准研究重点实验室培育基地,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2021

卷号：38

期号：1

起止页码：29

中文期刊名：甘肃中医药大学学报

外文期刊名：Journal of Gansu University of Chinese Medicine

基金：国家自然科学基金地区基金项目(81660625);国家重点研发计划项目(2017YFC1700705);甘肃省高校协同创新科技团队支持计划项目(2016C-05)。

语种：中文

中文关键词：当归;挥发油;气相色谱-质谱联用;化学组分;含量测定

外文关键词：Angelica sinensis;volatile oil;gas chromatography-mass spectrometry(GC-MS);chemical constituents;content determination

摘要：目的建立道地与近道地产区当归挥发油化学组分的分析方法。方法按照《中华人民共和国药典》2015年版四部2204通则挥发油提取法中的乙法提取挥发油,采用气相色谱-质谱(GC-MS)联用技术分析临洮当归与岷县当归挥发油化学组分。色谱柱为石英毛细管柱(30.00 m×250.00μm×0.25μm),检测器为氢火焰离子化检测器(FID),载气为高纯度氦气,进样口温度250℃,流速1.0 mL/min,进样量0.4μL,采用程序升温;EI离子源,电子能量70 eV,离子源温度230℃,全扫描,扫描范围30~500 m/z。结果临洮当归挥发油得率为1.38%,岷县当归为1.40%;从2个不同产地当归中共分离鉴定出32种化合物,其中岷县当归27种,临洮当归25种,各自占总挥发油含量的105.45%和98.22%,但2个产地中含量最高的均为(E)-藁本内酯,临洮当归为68.08%,岷县当归为66.06%;其最大差异为在临洮当归中发现了5种新化合物,分别为柠檬烯、4-十一碳烯、5,5-二甲基-1,3-环戊二烯、双环[4.1.0]庚-2-烯、环戊酮肟。结论该法初步测定了2个不同产地当归挥发油的化学组分,可为当归的引种及其栽培技术的制定提供参考,为评估新产区临洮当归药材的质量提供重要依据。
Objective To establish an analytical method for the chemical constituents of the volatile oil from Angelica sinensis in the authentic and near-authentic producing areas.Methods According to the general rule 2204 of Pharmacopoeia of The People’s Republic of China(Volume 4,2015 Edition),the volatile oil was extracted by method B of volatile oil extraction methods,and the chemical constituents thereof from Angelica sinensis produced in Lingtao and Min County were analyzed by gas chromatography-mass spectrometry(GC-MS).The chromatographic column was quartz capillary column(30.00 m×250.00μm×0.25μm),the detector was hydrogen flame ionization detector(FID),the carrier gas was high purity helium,the injector temperature at 250℃,the flow rate was 1.0 mL/min,the injection volume was 0.4μL,programmed temperature;EI ion source,the electron energy was 70 eV,the ion source temperature was 230℃,full scan,the scanning range is 30 to 500 m/z.Results The volatile oil extraction yield of Angelica sinensis from Lintao was 1.38%,that from Min County was 1.40%.Totally 32 compounds were isolated and identified from Angelica sinensis from two different producing areas,including 27 from Min County and 25 from Lintao,accounting for 105.45%and 98.22%of the total content of volatile oil,respectively.However,the highest content of(E)-ligustilide was found in the 2 producing areas,68.08%from Lintao and 66.06%from Min County.The biggest difference was that five new compounds were found in Angelica sinensis from Lintao,which were limonene,4-undecene,5,5-dimethyl-1,3-cyclopentadiene,bicyclo[4.1.0]hept-2-ene and cyclopentanone oxime.Conclusion This method has preliminarily determined the chemical constituents of the volatile oil from Angelica sinensis from 2 different producing areas,whereby it can provide a reference for the introduction and cultivation of Angelica sinensis,and provide an important basis for the evaluation of the quality of medicinal material of Angelica sinensis in new producing area Lintao.