文献类型：期刊文献

英文题名：HAVCR1 Affects the MEK/ERK Pathway in Gastric Adenocarcinomas and Influences Tumor Progression and Patient Outcome

作者：Xue, Ji[1];Li, Ying[2];Yi, Jianfeng[3];Jiang, Hong[4]

第一作者：Xue, Ji

通信作者：Jiang, H[1]

机构：[1]Second Hosp Jilin Univ, Dept Tradit Chinese Med, 218 Nanguan Dist, Changchun 130041, Jilin, Peoples R China;[2]Beijing Super Link Med Res Inst, Caihuying 58,Room 1102, Beijing 100000, Peoples R China;[3]Gansu Univ Chinese Med, Sch Med, Dept Surg, 35 Dingxi East Rd, Lanzhou 730000, Gansu, Peoples R China;[4]Xian Hosp Tradit Chinese Med, Dept Surg, 69 Fengcheng,8th Rd, Xian 710021, Shanxi, Peoples R China

第一机构：Second Hosp Jilin Univ, Dept Tradit Chinese Med, 218 Nanguan Dist, Changchun 130041, Jilin, Peoples R China

通信机构：[1]corresponding author), Xian Hosp Tradit Chinese Med, Dept Surg, 69 Fengcheng,8th Rd, Xian 710021, Shanxi, Peoples R China.

年份：2019

卷号：2019

外文期刊名：GASTROENTEROLOGY RESEARCH AND PRACTICE

收录：;Scopus(收录号：2-s2.0-85077110515);WOS:【SCI-EXPANDED(收录号:WOS:000503411500001)】；

基金：The authors acknowledge the support of the Gansu University Research Project (grant no. 2017A-052) and the Lanzhou Chengguan District Science and Technology Research Project (grant no. 2017 similar to 3-3).

语种：英文

摘要：The hepatitis A virus cellular receptor 1 (HAVCR1) gene as a sensitive and specific biomarker has been reported in various diseases. Especially, HAVCR1 overexpression promotes the development and progression of several human cancers. Hence, we aimed to detect the effects of HAVCR1 on gastric adenocarcinoma (GAC). We first determined the expression of HAVCR1 in GAC tissues compared with normal gastric tissues based on the Cancer Genome Atlas (TCGA) database using bioinformatics analysis methods. Then, we assessed the biological function of HAVCR1 in GAC cells using quantitative real-time reverse transcription-PCR (qRT-PCR), western blot, cell counting kit-8- (CCK-) 8, colony formation assay, wound healing assay, and transwell assay. Our results showed that HAVCR1 expression was upregulated in GAC tissues and positively associated with poor survival. Loss-of-function analyses indicated that knockdown of HAVCR1 inhibited the proliferation, colony formation, migration, and invasion of GAC cells. Furthermore, reduction of HAVCR1 in GAC cells can decrease the expression of phosphorylated MEK/ERK. These findings suggested that HAVCR1 may represent a potential biomarker for GAC prognosis, as well as a novel therapeutic target for GAC treatment.