文献类型：期刊文献

中文题名：层次分析-指标相关性赋权系数法赋权的响应面法优化黄芩提取工艺及其抗炎活性研究

英文题名：Optimization of extraction process of Scutellaria baicalensis Georgi by response surface methodology based on AHP-CRITIC weight assignment method and research on its anti-inflammatory activity

作者：黄钰芳[1,2,3];陈亚明[1];曹瑞[2,3];郭亚菲[2];曹强[2];杨成刚[2]

第一作者：黄钰芳

机构：[1]敦煌医学与转化教育部重点实验室,兰州730000;[2]甘肃中医药大学药学院,兰州730000;[3]西北中藏药省部共建协同创新中心,兰州730000

第一机构：敦煌医学与转化教育部重点实验室,兰州730000

年份：2023

卷号：32

期号：13

起止页码：1377

中文期刊名：中国新药杂志

外文期刊名：Chinese Journal of New Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：甘肃省中医药管理局科研基金项目(GZK-2019-20);敦煌医学与转化教育部重点实验室开放课题项目(DHYX20-13)。

语种：中文

中文关键词：黄芩;黄芩苷;响应面;层次分析-指标相关性赋权系数法;抗炎活性;工艺优化

外文关键词：Scutellaria baicalensis Georgi;baicalin;response surface;AHP-CRITIC;anti-inflammatory activity;process optimization

摘要：目的:确定指标权重,优化黄芩中黄酮类成分的超声提取工艺,并分析其体外抗炎活性。方法:在单因素实验基础上,选择乙醇浓度(A)、超声时间(B)、超声温度(C)及料液比(D)为考察因素,以黄芩苷、汉黄芩苷、黄芩素及汉黄芩素为考察指标,采用层次分析-指标相关性赋权系数法(AHP-CRITIC)确定各指标权重系数,结合响应面法拟合多元二项式,绘制三维图,选择最佳提取工艺;同时利用MTT法和Griess法评估黄芩提取物的体外抗炎活性。结果:黄芩中黄酮类成分的最佳提取条件为:乙醇浓度62.73%、超声时间32.23 min、超声温度51.15℃、料液比1∶16.12。3批验证样品的AHP-CRITIC综合评分平均值为85.28,与理论预测值的RSD为0.50%。MTT法结果表明黄芩提取物浓度>400μg·mL^(-1)时细胞活力显著下降(P<0.05,P<0.01);黄芩提取物浓度为50~350μg·mL^(-1)时,可显著抑制RAW264.7炎性细胞一氧化氮(NO)的释放(P<0.05,P<0.01)。结论:该方法稳定可靠,可用于黄芩中黄酮类成分的提取,其超声提取物具有一定的体外抗炎活性。
Objective:To determine the index weight coefficient,optimize the ultrasonic extraction process of flavonoids in Scutellaria baicalensis Georgi and analyze its anti-inflammatory activity in vitro.Methods:On the basis of single factor test,ethanol concentration(A),ultrasonic time(B),ultrasonic temperature(C) and material-to-liquid ratio(D) were selected as the investigation factors.Taking baicalin,wogonin,baicalein and wogonin as the inspection indicators,the AHP-CRITIC mixed weighting method was used to determine the weight coefficients of each index,multivariate binomial was fitted combined with the response surface method,a three-dimensional graph was drawn,and the best extraction process was chosen.At the same time,the MTT method and Griess method were used to evaluate the in vitro anti-inflammatory activity of the ultrasonic extraction of Scutellaria baicalensis Georgi.Results:The optimal extraction conditions for flavonoids in Scutellaria baicalensis Georgi were as follows:ethanol concentration of 62.73%,ultrasonic time of 32.23 min,ultrasonic temperature of 51.15 ℃,and material-to-liquid ratio of 1∶16.12.The average AHP-CRITIC comprehensive score of three batches of verification samples was 85.28,and the RSD with the theoretical prediction value was 0.50%.The results of MTT method showed that when the concentration of Scutellaria baicalensis Georgi extraction was greater than 400 μg·mL^(-1),the cell viability decreased significantly(P<0.05,P<0.01).When the concentration of Scutellaria baicalensis Georgi extraction was 50~350 μg·mL^(-1),the release of NO in RAW264.7 inflammatory cells was significantly inhibited(P<0.05,P<0.01).Conclusion:The method is stable and reliable and can be used for the extraction of flavonoids from Scutellaria baicalensis Georgi,and the ultrasonic extract has strong anti-inflammatory activity in vitro.