文献类型：期刊文献

中文题名：黄芪乌梅配方颗粒对GK大鼠胰岛素抵抗的影响

英文题名：Effect of Huangqi-Wumei granule on insulin resistance in GK rats

作者：刘斌[1];李淑玲[2];刘凯[1];李应东[1]

第一作者：刘斌

机构：[1]甘肃中医药大学中西医结合学院;[2]甘肃中医药大学附属医院省级重点实验室

第一机构：甘肃中医药大学中西医结合学院

年份：2016

卷号：41

期号：12

起止页码：987

中文期刊名：解放军医学杂志

外文期刊名：Medical Journal of Chinese People's Liberation Army

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：甘肃省中医药管理局科研立项课题(GZK-2016-35)~~

语种：中文

中文关键词：黄芪乌梅配方颗粒;大鼠;GK;胰岛素抗药性

外文关键词：Huangqi-Wumei granule; rats, GK; insulin resistance

摘要：目的观察黄芪乌梅配方颗粒对GK大鼠胰岛素抵抗的影响。方法 4周龄雄性GK大鼠32只,随机分为模型组、阳性对照组(盐酸二甲双胍肠溶片0.15g/kg)、黄芪乌梅低剂量组(黄芪乌梅配方颗粒1.3g/kg)、黄芪乌梅高剂量组(黄芪乌梅配方颗粒5.2g/kg),每组8只;同时以8只同周龄雄性Wistar大鼠为空白组。灌胃治疗10周,分别于0、2、4、6、8、10周测量大鼠体重、空腹血糖。干预第10周末取血,测量空腹血清胰岛素(FINS)和糖化血红蛋白(GHb)水平,计算胰岛素抵抗指数(HOMA-IR),然后急性失血法处死大鼠,取肝脏组织,蛋白印迹法检测肝脏胰岛素受体(IR)和胰岛素受体底物-1(IRS-1)蛋白的表达。结果干预10周后,空白组、模型组、阳性对照组大鼠体重差异无统计学意义(P>0.05),而各黄芪乌梅干预组大鼠体重明显减轻(P<0.05)。与空白组相比,模型组大鼠空腹血糖明显升高(P<0.05);与模型组相比,各黄芪乌梅干预组及阳性对照组大鼠空腹血糖明显降低(P<0.05)。与空白组相比,模型组大鼠FINS、GHb和HOMA-IR明显升高(P<0.05);与模型组相比,各黄芪乌梅干预组和阳性对照组大鼠HOMA-IR均降低(P<0.05),黄芪乌梅高剂量组和阳性对照组大鼠FINS、GHb降低(P<0.05)。与空白组相比,模型组IR和IRS-1蛋白表达明显下降(P<0.05);与模型组相比,各黄芪乌梅干预组及阳性对照组IR和IRS-1蛋白表达均明显增加(P<0.05)。结论黄芪乌梅配方颗粒能降低GK大鼠血糖,减轻体重,改善大鼠胰岛素抵抗,其机制可能与促进肝脏组织中IR和IRS-1蛋白表达有关。
Objective To observe the effect of Huangqi-Wumei （Mongolian milkvetch-Smolced plum, HW） granule on insulin resistance in GK rats. Methods Thirty-two male 4-week-old GK rats were randomly divided into four groups （8 each）： model group, positive control group （metformin hydrochloride enteric-coated tablets, 0.15g/kg）, low dose HW granule group （HW granule 1.3g/kg） and high dose HW granule group （HW granule 5.2g/kg）; meantime 8 male Wistar rats of eight-week-old were used as normal control group （normal saline）. During 10 weeks of garage, multiple measurements of rats＇ body weight and fasting blood glucose （FBG） were detected respectively on the 0, 2, 4, 6, 8 and 10 week. Fasting serum insulin （FINS） and glycosylated hemoglobin （GHb） were measured and insulin resistance index （HOMA-IR） was calculated at the end of the 10th week. Rats were sacrificed by acute blood loss, and the expressions of insulin receptor （IR） and insulin receptor substrate-1 （IRS-1） protein in rats＇ liver were measured by Western blotting. Results Ten weeks after intervention, compared with the normal control group, no significant difference of the body weight existed in model group （P〉0.05） and positive control group （P〉0.05）, but the body weight in HW granule groups reduced significantly （P〈0.05）. Compared with the normal control group, the FBG, GHb and HOMA-IR in the model group was significantly increased （P〈0.05）; compared with the model group, the HOMA-IR in HW granule groups and positive control group decreased significantly （P〈0.05）, the FINS and GHb in the high dose HW granule group and the positive control group decreased （P〈0.05）. Compared with the normal control group, the expression of IR and IRS-1 protein in the model group decreased （P〈0.05）. Compared with the model group, the expression of IR and IRS-1 protein in the HW granule groupsand the control group increased significantly （P〈0.05）. Conclusions HW granule may reduce the blood glucose, lose weight and attenuate insulin resistance of GK rats. The mechanism may be related to the increased expression of IR and IRS-1 protein in liver tissue of GK rats.