文献类型：期刊文献

英文题名：Evaluation of antiviral activity of organic-polyoxometalate hybrids based on berberine against encephalomyocarditis virus in vitro

作者：Tan, Chunxia[1];Zhao, Jieying[1];Huang, Zizhen[1];Hou, Jixia[1];Zhang, Tengyu[1];Xie, Jingying[2];Feng, Ruofei[2]

第一作者：谈春霞

通信作者：Tan, CX[1];Feng, RF[2]

机构：[1]Gansu Univ Chinese Med, Lanzhou 730030, Peoples R China;[2]Northwest Minzu Univ, Biomed Res Ctr, Key Lab Biotechnol & Bioengn, State Ethn Affairs Commiss, Lanzhou 730030, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou 730030, Peoples R China;[2]corresponding author), Northwest Minzu Univ, Biomed Res Ctr, Key Lab Biotechnol & Bioengn, State Ethn Affairs Commiss, Lanzhou 730030, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

卷号：4

期号：4

外文期刊名：POLYOXOMETALATES

收录：EI(收录号：20260519983473);WOS:【ESCI(收录号:WOS:001652127600001)】；

基金：This work was supported by Open Funds of the Biomedical Research Center from Northwest Minzu University (No. BRC-KF202303), Fundamental Research Funds for the Central Universities (No. 31920240105), and the National Natural Science Foundation of China (No. 32260037).

语种：英文

外文关键词：polyoxometalates; berberine; antiviral activity; BHK-21; biosynthesis stage; encephalomyocarditis virus (EMCV)

摘要：Polyoxometalates are promising inorganic drugs with antiviral activity. However, they pose a risk to human because of their potential accumulation in the body. Polyoxometalates encapsulated with berberine from a traditional Chinese herb may exhibit lower cytotoxicity. In this study, the antiviral effects of four berberine-based organic-polyoxometalate hybrids (BR-POMs) on BHK-21 and PK-15 cells were evaluated in vitro using encephalomyocarditis virus (EMCV) or pseudorabies virus (PRV) models. The collected cells were used for quantitative polymerase chain reaction analysis. The supernatants were collected to quantify the viral loads using a tissue culture infectious dose (TCID50) assay in vitro. EC50 and CC50 were determined through dose-response experiments, and the EC50/CC50 ratio was used as a selectivity index to measure the antiviral activity. The results demonstrate that all BR-POMs exhibited certain antiviral activity. The BR-POMs did not exert toxicity against the EMCV- or PRV-infected cells at the tested concentration (CC50 > 40 mu M). Notably, BR-EuSiW (EC50 15.07 mu M, CC50 651.2 mu M, SI 43.21) exerted antiviral effects by acting on the virus at its biosynthesis stage, thereby inhibiting virus proliferation in a dose-dependent manner. This study demonstrates that organic-polyoxometalate hybrids represent a new strategy for developing antivirals against EMCV.