文献类型：期刊文献

英文题名：Antioxidant activity and potential ameliorating effective ingredients for high altitude-induced fatigue from Gansu Maxianhao (Pedicularis Kansuensis Maxim.)

作者：Yu Lijun[1];Cao Xinyuan[2,3,4];Tao Wendi[2,3,4];Li Maoxing[2,3,4,5];Li Xiaolin[2,3,6];Chen Liping[2,3]

第一作者：Yu Lijun

通信作者：Li, MX[1];Li, MX[2];Li, MX[3]

机构：[1]Gansu People Hosp PLA, Dept Pharm, Lanzhou 730000, Peoples R China;[2]940th Hosp Joint Logist Support Force PLA, Dept Pharm, Lanzhou 730050, Peoples R China;[3]Key Lab Prevent & Cure Plateau Environm Damage PL, Lanzhou 730050, Peoples R China;[4]Lanzhou Univ, Dept Pharm, Lanzhou 730000, Peoples R China;[5]Gansu Univ Chinese Med, Dept Pharm, Lanzhou 730000, Peoples R China;[6]Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China

第一机构：Gansu People Hosp PLA, Dept Pharm, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Dept Pharm, Lanzhou 730000, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Dept Pharm, Lanzhou 730000, Peoples R China;[3]corresponding author), Lanzhou Gen Hosp PLA, Key Discipline Tradit Chinese Med, Dept Pharm, Lanzhou 730000, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2020

卷号：40

期号：1

起止页码：83

外文期刊名：JOURNAL OF TRADITIONAL CHINESE MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000518866300009)】；

基金：Supported by Project of Traditional Chinese Medicine Administration, Gansu Province: Study on the Active Constituents of Tibetan Medicine Pedicularis to Improve Plateau Exercise Efficiency (GZK-2015-59); Project of Military Medical and Health Research, PLA: Exploring the Role and Mechanism of the Prevention of High Altitude Cerebral Edema by Tibetan Medicine Pangxiejia (P. Kawaguchii Murata) Based on Inflammatory Response Induced by Hypobaric Hypoxia (CLZ15JA05, CLZ15JB04)

语种：英文

外文关键词：Pedicularis; Glycosides; Antioxidants; Fatigue; Hypoxia

摘要：OBJECTIVE: To investigate the antioxidant property and potential ameliorating effective ingredients for high altitude-induced fatigue from Gansu Maxianhao (Pedicularis Kansuensis Maxim.). METHODS: Macroporous adsorptive resin combined with polyamide chromatographic column was used to obtain water extract (P-1), high polar part (P-2), iridoid glycosides part (P-3) and phenylethanoid glycosides part (P-4) of Gansu Maxianhao (Pedicularis Kansuensis Maxim.). Antioxidant activity of each part was investigated employing a series of in vitro models. Hematoxylin-eosin staining, analysis of blood biochemical parameters, along with molecular analyses examining oxidative stress makers, metabolite, metabolic enzyme and energy substance in liver, skeletal muscle and/or serum were further measured. RESULTS: The results showed phenylethanoid glycosides (PhGs) exhibited more effective with 1,1-diphenyl-2-picrylhydrazyl radical scavenging ability, 2-2-Azinbis (3-ehtylbenzothiazolin-6-sulfnicAcid) Diammonium Salt scavenging activity, hydroxyl radical scavenging activity, reducing ability and antioxidant activity to varying extents. Under a hypobaric hypoxia attack in a hypobaric chamber (8000 m, 7 consecutive days), PhGs was administered to BALB/C mice at doses of 50, 200, 400 mg/kg and antifatigue property was evaluated using a swimming test at an altitude of 4000 m. The results showed that PhGs of Gansu Maxianhao (Pedicularis Kansuensis Maxim.) could significantly prolong the burden swimming time of mice, reduce the hypoxia-induced oxidative stress, remove the accumulated products of metabolism, improve the energy metabolism as well as improve preservation of endogenous glycogen stores. CONCLUSION: The ameliorating effect against altitude-induced fatigue of PhGs from Gansu Maxianhao (Pedicularis Kansuensis Maxim.) might come from the alleviation of oxidative stress, reduction of the adverse metabolic products, normalizing energy metabolism and increasing energy substances reserves. PhGs is a potential antioxidant and novel remedy for fatigue due to high-altitude hypoxia. (C) 2020 JTCM. All rights reserved.