文献类型：期刊文献

中文题名：痛泻要方对溃疡性结肠炎大鼠结肠黏膜ERK1、ERK2基因表达的影响

英文题名：Influence of Tongxie Yao Fang on ERK1 and ERK2 Gene Expressions of Colonic Mucosa in Rats with Ulcerative Colitis

作者：朱向东[1];段永强[1];王燕[1];汪斌[1];曹燕飞[1]

第一作者：朱向东

机构：[1]甘肃中医学院基础课部,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2013

卷号：29

期号：4

起止页码：347

中文期刊名：南京中医药大学学报

外文期刊名：Journal of Nanjing University of Traditional Chinese Medicine

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2013_2014】；

基金：国家自然科学基金地区基金(81060283)

语种：中文

中文关键词：痛泻要方;溃疡性结肠炎;基因表达

外文关键词：Tongxie Yao Fang; ulcerative colitis; gene expression

摘要：目的观察痛泻要方对溃疡性结肠炎(UC)模型大鼠结肠黏膜ERK1、ERK2基因表达的影响,探讨其作用机制。方法将实验动物分为6组,采用TNBS/乙醇灌肠法造模,肉眼观察结肠大体形态损伤并进行评分,以大鼠结肠黏膜ERK1、ERK2为观察指标,采用RT-PCR法检测ERK1、ERK2基因表达水平。结果肉眼观察模型组大鼠结肠组织黏膜层可见炎症和溃疡形成,与空白组比较,P<0.05,证实模型成功。模型组大鼠结肠组织ERK1、ERK2基因的相对表达量均高于空白组(P<0.05);治疗后,痛泻要方高剂量组、中剂量组ERK1、ERK2基因的相对表达量均较模型组升高(P<0.05,P<0.01)。结论痛泻要方对TNBS/乙醇法UC大鼠模型结肠黏膜ERKl、ERK2基因的相对表达量有上调作用,提示痛泻要方治疗作用可能与ERK信号转导途径被激活有关。
OBJECTIVE To observe the influence of Tongxie Yao Fang on the ERK1 and ERK2 gene expressions of colonic mucosa in rats with ulcerative colitis （UC）,and to identify its underlying mechanism of action.METHODS Experimental animals were divided into six groups and prepared by means of TNBS/ ethanol enema to observe their morphological injury and damage with the naked eyes,and to detect ERK1 and ERK2 gene expressions by means of RT-PCR with ERK1 and ERK2 being the observation targets.RESULTS Inflammation and ulceration on the colonic mucous membrane of rats were observed with naked eyes,which suggested a successful modeling compared with the control group （P＜0.05）.The relative expression amount of ERK1 and ERK2 of colonic tissues of rats in the model group was higher than that of the blank group （P＜0.05） ;after treatment,compared with the model group,the relative expression amount of ERK1 and ERK1 in the prescription highand moderate-dose groups were increased （P＜0.05 and P＜0.01）.CONCLUSION Tongxie Yao Fang can promote the relative expression amount of ERK1 and ERK2 of colonic tissues of rats with UC,which suggests the therapeutic effects of the prescription are probably related to the activation of ERK signal transduction pathway.