文献类型：期刊文献

中文题名：代谢综合征大鼠模型的建立及评价

英文题名：Establishment and Evaluation of the Metabolic Syndrome Models in Rats

作者：李耀华[1];谢萍[1];王娟[1]

第一作者：李耀华

机构：[1]甘肃中医药大学临床医学院,兰州730000

第一机构：甘肃中医药大学临床医学院

年份：2017

卷号：46

期号：4

起止页码：86

中文期刊名：医学研究杂志

外文期刊名：Journal of Medical Research

收录：CSTPCD

基金：甘肃省卫生行业科研计划项目(GSWSKY-2015-04)

语种：中文

中文关键词：代谢综合征;大鼠模型;链脲佐菌素

外文关键词：Metabolic syndrome; Rat models; Streptozotocin

摘要：目的复制与人类代谢综合征(MS)相似的大鼠模型,确定成模标准并评价成模率,为研究MS发病机制及评价药物疗效提供实验基础。方法 SPF级雄性7周龄Wistar大鼠随机分为正常组(n=10)和造模组(n=70),分别予正常饮食和高脂高糖高盐饮食饲养16周。每4周测量各组体重、腹围、体长、血压及空腹血糖(FPG),饲养至15周时,造模组一次性腹腔注射链脲佐菌素(STZ)35mg/kg,1周后检测各组大鼠空腹血清胰岛素(FINS)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C),计算稳态模型胰岛素抵抗指数(HOMA-IR),评价造模效果。结果随着造模时间的逐渐延长,造模组大鼠体重、腹围、血压、FPG、TG、TC、FINS、HOMA-IR及LDL-C水平均高于正常组(P<0.05),HDL-C低于正常组(P<0.05),体长两组比较差异无统计学意义(P>0.05),MS大鼠成模率为74.3%。结论高脂高糖高盐饮食诱导联合一次性小剂量腹腔注射STZ可成功复制MS大鼠模型,符合MS的病理生理特征,此方法操作简便,成模率高,可作为研究MS及药物干预疗效的理想模型。
Objective To replicate the rats model of human metabolic syndrome （MS） , determine the standard of the model, evaluate the success rate of model, provide the experimental basis for the study of the pathogenesis and therapeutic study of MS. Methods Eighty specific pathogen free male Wistar rats aged 7 weeks were randomly divided into the normal group （ n = 10） and the experimental group （n = 70）, which were respectively fed with normal diet and high fat, high sugar, high salt diet for 16 weeks. The rats＇ body weight, abdominal circumference, body length, blood pressure and fasting plasma glucose （FPG） were measured every 4 weeks. The experimental group rats were injected with streptozocin（STZ） 35mg/kg intraperitoneally once at the fifteenth week, the blood lipids （TC, TG, LDL- C and HDL- C） and FINS were measured, and HOMA-IR was calculated a week late. Results With the feeding time gradually extended, the rats＇ body weight, abdominal circumference, blood pressure, FPG, TG, TC, FINS, HOMA - IR and LDL - C levels were all significantly higher in the experimental group than in the normal group （ P 〈 0.05 ） , while HDL - C was lower than that of the normal group （P 〈 0.05 ）. There was no significant difference in body length between the two groups（ P 〉 0.05 ）. The success rate of MS rats models were 74.3%. Conclusion High fat, high sugar, high salt diet combined with a small dose of STZ intraperitoneal injection can successfully replicate the rat model of MS. This kind of rat models conforms to the pathological characteristic of MS, which can be easily operated and with high success rate. Therefore, it could be applied as an ideal MS animal model for the study of MS and drugs therapeutic effect.