文献类型：期刊文献

英文题名：HPV16-miR-H1 promotes proliferation, migration, and glycolysis in head and neck squamous cell carcinoma cells via the PTEN/AKT pathway

作者：Wang, Ruilin[2];Li, Shangdong[1];Ma, Yuxiu[1];Da, Dezhuan[2];Zhang, Qi[1];Dang, Chunyan[2];Zhao, Xuhui[2];Liang, Shuang[1];Li, Hongling[2]

第一作者：Wang, Ruilin

通信作者：Li, HL[1]

机构：[1]Gansu Univ Tradit Chinese Med, Clin Med Coll 1, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Prov Hosp, Dept Oncol, 204 Donggang West Rd, Lanzhou 73000, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Prov Hosp, Dept Oncol, 204 Donggang West Rd, Lanzhou 73000, Gansu, Peoples R China.

年份：2025

卷号：15

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：;Scopus(收录号：2-s2.0-105026218563);WOS:【SCI-EXPANDED(收录号:WOS:001651184200004)】；

基金：National Health and Health Commission Key Laboratory Fund Project "Study on the immune escape mechanism of EBV-associated gastric cancer based on the EBV-miR-BART5-enhancer-PDL1/PD1 axis" (NHCDP2022005); Natural Science Foundation of Gansu Province Project "EBV virus-derived miRNA-BART18-3P participates in gastric cancer development through the adipose de novo synthesis pathway Participation in Gastric Cancer Development" (24JRRA586); Gansu Provincial Science and Technology Department Joint Fund Project "Key Molecular Mechanisms and Clinical Diagnosis and Treatment of Colorectal Cancer Occurrence, Recurrence, and Metastasis" (23JRRA1545); Gansu Provincial People's Hospital 2022 Master's Degree/Postdoctoral Fund Project "HPV16-miR-1 Promotes Tongue Squamous Carcinoma Proliferation and Metastasis via Regulating CERK/PI3K/AKT Axis Study on the role of HPV16-miR-1 in promoting the proliferation and metastasis of tongue squamous carcinoma through regulating the CERK/PI3K/AKT axis" (22GSSYD-44).

语种：英文

外文关键词：Head and neck squamous cell carcinoma; Human papillomavirus; Virus-encoded MicroRNAs; HPV16-miR-H1; Glycolysis

摘要：Human papillomavirus type 16 (HPV16) is the most common type of infection in head and neck squamous cell carcinoma (HNSCC).The HPV16-encoded miRNA, HPV16-miR-H1, has been validated in cervical cancer tissues and cells, but its role in the pathogenesis of HNSCC remains unclear. The expression level of HPV16-miR-H1 in HNSCC cells was detected, and its mechanism of action was analyzed using bioinformatics tools and RNA sequencing. Overexpression and knockdown of HPV16-miR-H1 were performed to validate its regulatory effects on the PTEN/AKT axis and its impact on cell proliferation, migration, the cell cycle, and glycolysis. HPV16-miR-H1 was highly expressed in HPV16-positive HNSCC cells. Bioinformatics prediction and gene enrichment analysis indicated that HPV16-miR-H1 regulates PTEN transcription and the glycolysis process. Dual-luciferase reporter assays further confirmed that HPV16-miR-H1 targets the 3'-UTR region of PTEN. Functional experiments demonstrated that HPV16-miR-H1 affects HNSCC cell proliferation, migration, the cell cycle, and glycolysis. The expression of HPV16-miR-H1 in HNSCC cells was validated for the first time. Further studies revealed that it regulates the PTEN/AKT axis by targeting the 3'UTR region of PTEN, thereby enhancing the proliferation, migration, and glycolytic processes in HNSCC cells.