文献类型：期刊文献

中文题名：丁苯酞对哮喘小鼠气道黏液高分泌及IL-13、TNF-α的影响

英文题名：Effects of Butylphthalide on airway mucus hypersecretion,IL-13 and TNF-α in asthmatic mice

作者：庞亚蓉[1];杜玥[1];王志旺[1];席建宏[1];李济阳[1];韦凌霞[1];丁茂鹏[1];程小丽[1]

第一作者：庞亚蓉

机构：[1]甘肃中医药大学,兰州730000

第一机构：甘肃中医药大学

年份：2021

卷号：37

期号：5

起止页码：557

中文期刊名：中国应用生理学杂志

外文期刊名：Chinese Journal of Applied Physiology

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；PubMed;

基金：国家自然科学基金项目(81460668);甘肃省自然科学基金项目(20JR5RA183,1606RJZA011,1310RJZA086);甘肃省中医药研究中心开放基因课题(ZYZX-2020-ZX4)。

语种：中文

中文关键词：丁苯酞;哮喘;黏液高分泌;白介素-13(IL-13);肿瘤坏死因子-α(TNF-α);小鼠

外文关键词：Butylphthalide(NBP);asthma;mucus hypersecretion;interleukin-13(IL-13);tumor necrosis factor-α(TNF-α);mouse

摘要：目的:研究丁苯酞对哮喘小鼠气道黏液高分泌及白介素-13(IL-13)、肿瘤坏死因子-α(TNF-α)的影响。方法:小鼠随机分为空白对照组、模型对照组、阳性对照组和丁苯酞高、中、低剂量(100、50、25 mg/kg)组(n=12)。实验第1日、8日、15日通过注射卵清白蛋白(OVA)致敏,第22日吸入OVA连续激发5周复制哮喘模型,同时在激发前给予丁苯酞20 mg/kg进行干预,观测哮喘行为学、气道杯状细胞及黏蛋白5ac(Muc5ac)的分泌,测定支气管肺泡灌洗液(BALF)粘度,采用ELISA法测定BALF中Muc5ac、IL-13及TNF-α的水平。结果:与空白对照组比较,模型对照组小鼠打喷嚏、抓鼻及哮喘的程度显著加重(P<0.01),小鼠气道上皮杯状细胞增生及Muc5ac的分泌显著增加(P<0.01),BALF的粘度及其中的Muc5ac、IL-13和TNF-α的含量显著升高(P<0.01);与模型对照组比较,25、50、100 mg/kg丁苯酞干预后哮喘行为学评分明显下降(P<0.01);小鼠气道上皮杯状细胞增生、Muc5ac的分泌、BALF的粘度及其中的Muc5ac、IL-13和TNF-α的含量均明显降低(P<0.05,0.01)。结论:丁苯酞具有抑制哮喘气道黏液高分泌而平喘的作用,缓解IL-13、TNF-α异常高表达是其抑制气道黏液高分泌的作用机制之一。
Objective:To investigate the effects of Butylphthalide(NBP)on airway mucus hypersecretion,interleukin-13(IL-13)and tumor necrosis factor-α(TNF-α)in asthmatic mice.Methods:The mice were randomly divided into control group,asthma group,DEX group and high,medium and low doses of NBP(100,50,25 mg/kg)groups(n=12).Ovalbumin(OVA)injection was sensitized on the 1st,8th,and 15th day of the experiment,and OVA was inhaled on the 22nd day to stimulate for 5 weeks to replicate the asthma model,and 20 mg/kg of NBP was given for intervention before the challenge.Finally,the asthma behavior,the secretion of goblet cells and Mucin 5ac(Muc5ac)were observed,and meanwhile the viscosity of bronchoalveolar lavage fluid(BALF)and the levels of Muc5ac,IL-13 and TNF-αin BALF were measured by ELISA.Results:Compared with the control group,the degree of sneezing,nose scratching and asthma,the proliferation of airway epithelial goblet cells and secretion of Muc5ac in the asthma group were increased significantly(P<0.01),meanwhile,the viscosity of BALF and the contents of Muc5ac,IL-13 and TNF-αwere also increased significantly(P<0.01).Compared with the asthma group,the above behavioral scores of asthma were decreased significantly(P<0.01)after the intervention of 25,50 and 100 mg/kg NBP,as well as the proliferation of airway epithelial goblet cells,secretion of Muc5ac,the viscosity of BALF and the contents of Muc5ac,IL-13 and TNF-αwere significantly lower than those of the asthma group(P<0.05,0.01).Conclusion:NBP has the effect of anti-asthma by inhibiting mucus hypersecretion,and one of its mechanisms is to alleviate the abnormal expressions of IL-13 and TNF-α.