文献类型：期刊文献

中文题名：气相色谱-质谱联用同时测定龙脑樟鲜叶中右旋龙脑、樟脑及异龙脑的含量

英文题名：Simultaneous determination of contents of D-borneol,camphor and isoborneol in fresh leaves of Cinnamomum Camphora chvar.Borneol by GC-MS

作者：荔淑楠[1];王引权[1,2];徐名刷[3];李建文[3];张卫平[3];王彤彤[1];樊秦[1];雒军[1];夏琦[1]

第一作者：荔淑楠

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]甘肃省中药质量与标准研究重点实验室,甘肃兰州730000;[3]广东万森生态科技发展有限公司韶关市南岭珍稀药用植物工程技术研究开发中心,广东乳源512700

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2018

卷号：35

期号：5

起止页码：32

中文期刊名：甘肃中医药大学学报

外文期刊名：Journal of Gansu University of Chinese Medicine

基金：国家自然科学基金地区基金项目(8166062581;26061681;060327);甘肃省高校协同创新科技团队支持计划项目(2016C-05FF09)

语种：中文

中文关键词：龙脑樟鲜叶;右旋龙脑;樟脑;异龙脑;气相色谱-质谱;含量测定

外文关键词：fresh leaves of Cinnamomum Camphora chvar. Borneol;D-borneol;Camphor;Isoborneol;GC-MS;content determination

摘要：目的建立气相色谱-质谱(GC-MS)联用同时测定龙脑樟鲜叶中右旋龙脑、樟脑及异龙脑含量的方法。方法采用超声辅助水蒸气蒸馏法提取龙脑樟鲜叶,60%乙醇重结晶,GC-MS法测定右旋龙脑、樟脑及异龙脑的含量。色谱条件:色谱柱为石英毛细管柱(30.00 m×250.00μm×0.25μm),载气为高纯氦气,纯度≥99.999%,进样量1μL,柱温从60℃开始程序升温,流速1 mL/min,进样口温度280℃。质谱条件:电子能量70 eV,离子源温度230℃,溶剂切除时间5.0 min,扫描范围50～650 m/z,扫描模式为全扫描。结果右旋龙脑、樟脑及异龙脑的回归方程分别为Y=2.0×10~8X+1.0×10~8(r=0.999 9),Y=7.0×107X-3.0×107(r=0.999 9),Y=8.0×107X-8. 0×107(r=0.999 9),表明三者分别在0.03～0.27μg,0.06～0.48μg,0.05～0.36μg范围内线性关系良好。三者的平均回收率分别为96. 32%,95. 49%和97. 51%,RSD分别为1. 92%,0.85%和1.87%。从龙脑樟鲜叶中提取纯化后的右旋龙脑含量百分比均在98.09%以上,樟脑含量百分比较低,不含异龙脑。结论该法操作简便、准确,灵敏度、精密度高,分析速度快,可作为龙脑樟药材的质量控制,并为龙脑樟药材的综合利用提供了科学依据。
Objective To establish a GC-MS method for simuhaneous determination of D-borneol, camphor and isoborneol contents in fresh leaves of Cinnamomum Camphora chvar. Borneol. Methods Ultrasound-assisted steam distillation was used to extract fresh leaves of Cinnamomum Camphora chvar. Borneol with 60% ethanol recrystallization,contents of D-borneo1, camphor and isoborneol were were determined by GC-MS assay. Chromato- graphic conditions：chromatographic column was silica capillary column （30.00 m ×250.00 μm×0.25 μm）, initial temperature 60 ℃, carrier gas was high purity helium, purity ≥ 99.999%, flow rate was 1 mL/min, injection volume was 1 μL;Inlet temperature was 280 ℃ ;mass spectrometry conditions：EI ion source was electron energy 70 eV,ion source temperature was 230 ℃, solvent removal time was 5.0 min, scanning mode was full scan, scanning range was 50 to 650 m/z.Results The respective regression equations of D-borneol, Camphor, and Isoborneol were Y= 2.0×10^8X＋ 1.0×10^8（r=0.999 9）,Y=7.0×10^7X-3.0×10^7（r=0.999 9）,Y=8.0×10^7X-8.0×10^7（r=0.999 9）.They showed a good linear relationship at the range of 0.03 to 0,27 μg,0.06 to 0.48 μg,0.05 to 0.36μg.The respective average recovery was 96.32% ,95.49% ,97.51% ,while RSD was 1.92% ,0.85% ,1.87% respectively.Mter extraction and purification,the percentage composition of Borneol was more than 98.09% ,that of camphor was low,with no isoborneol.Coneluslon The experimental method is simple, accurate, sensitive, precise and rapid,which can be used for quality control of Cinnamomum Camphora chvar. Borneol and can provide a scientific basis for its comprehensive utilization.