文献类型：期刊文献

中文题名：RP-HPLC法同时测定雪松松针中杨梅素、槲皮素和山柰酚的含量

英文题名：Simultaneous determination of myricetin,quercetin and kaempferol in pine needles of Cedrus deodara by RP-HPLC

作者：石晓峰[1];刘东彦[1];李爽[2];白朝辉[2]

第一作者：石晓峰

机构：[1]甘肃省医学科学研究院;[2]甘肃中医学院

第一机构：甘肃省医学科学研究院

年份：2012

卷号：32

期号：9

起止页码：1550

中文期刊名：药物分析杂志

外文期刊名：Chinese Journal of Pharmaceutical Analysis

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：甘肃省技术研究与专项计划项目(编号:0709TCYA022);甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金项目(zzy-2011-05);甘肃省卫生厅科研计划项目(WST07-02)

语种：中文

中文关键词：雪松松针;黄酮类化合物;杨梅素;槲皮素;山柰酚;喜马拉雅杉(香柏)含量测定;反相高效液相色谱

外文关键词：pine needles of Cedrus deodara ; flavonoids ; myricetin ; quercetin ; kaempferol ; Ximalayashan （Xiangbai）assay ; RP - HPLC

摘要：目的:建立同时测定雪松松针中3个黄酮类化合物杨梅素、槲皮素和山柰酚含量的反相高效液相色谱法。方法:样品用50倍60%甲醇回流提取1 h,提取液加10%盐酸水解1 h。采用TC-C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,流速1.0 mL.min-1,检测波长360 nm,柱温30℃。结果:雪松松针中杨梅素、槲皮素和山柰酚3个黄酮类化合物浓度在0.4～8.0μg.mL-1、0.5～9.5μg.mL-1和0.8～15.2μg.mL-1范围内呈良好线性关系,相关系数在0.9998～0.9999之间;加样回收率(n=9)分别为98.4%,97.5%,98.9%。结论:该方法简单、快速、高效,可用于雪松松针中黄酮类化合物的测定。
Objective： To develop a RP - HPLC method for the simultaneous determination of three flavonoids （ myr- icetin, quercetin, kaempferol） in pine needles of Cedrus deodara. Methods： The samples were pretreated, including reflux extraction with 60% methanol for 1 h（ with the ratio of liquor to material of 50：1 ） ,and then extracting solu- tion hydrolysis with 10% hydrochloric acid for another 1 h. Agilent TC -C1s （250 mm x 4.6 mm,5 p,m） column was adopted;The mobile phase was acetonitrile -0.1% phosphoric acid solution with the gradient elution at a flow rate of 1.0 mL . min 1 ;The column temperature was 30℃ and the detection wavelength was μg· mL-1p^g ~ mL- ~ for myricetin,0.5 - 9.5μg· mL-1 for quercetin and 0.8 - 15.2μg· mL-1for kaempferol, respectively. The correlation coefficients were between 0. 9998 -0. 9999. The average recoveries （n =9） of the three flavonoids were 98.4% ,97.5% and 98.9% ,respectively. Conclusion： The method is simple, rapid and efficient, which is applicable for determination of flavonoids in pine needles of C. deodara.