文献类型：期刊文献

英文题名：Glutamate-cysteine ligase catalytic and its modifier function as novel immunotargets in gastric adenocarcinoma

作者：Da, Dezhuan[1];Pan, Zhiang[1];Zeng, Lu[1];Dang, Yamei[1];Dang, Chunyan[1];Huang, Yunxia[1];Shi, Dujuan[1];Li, Hongling[1]

第一作者：Da, Dezhuan

通信作者：Li, HL[1]

机构：[1]Gansu Univ Chinese Med, Gansu Prov Hosp, Dept Oncol, Clin Med Coll 1, 204 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Gansu Prov Hosp, Dept Oncol, Clin Med Coll 1, 204 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2023

卷号：46

期号：1

起止页码：143

外文期刊名：ASIAN JOURNAL OF SURGERY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000898075500021)】；

基金：This study was sponsored by grants from The National Natural Science Foundation of China (No.81760537, 81560498) , The International Cooperation Project of Department of science and technology of Gansu Province (No. 20YF8WA096) , and The Hospital Internal Scientific Research Foundation of Gansu Provincial Hospital (No. 19SYPYA-3) .

语种：英文

外文关键词：Immunohistochemistry; Gastric adenocarcinoma; GCLC; GCLM

摘要：Objectives: To determine the expression and function of glutamate-cysteine ligase catalytic (GCLC) and glutamate-cysteine ligase catalytic modifier (GCLM) in gastric adenocarcinoma.Methods: Bioinformatics was used to analyze the expression of GCLC and GCLM. We download and analyzed the expression of gastric adenocarcinoma patients from TCGA database. Moreover, the method of immunochemistry was used to verify the expression of GCLC and GCLM in gastric adenocarcinoma.Results: At first, the expression of GCLC and GCLM in gastric adenocarcinoma tissues were both signif-icantly higher compared with normal tissues analyzed via TCGA database. Then, gastric adenocarcinoma tissues were collected and performed with immunochemistry. The gastric adenocarcinoma with positive staining for GCLC and GCLM was 77% and 80%, respectively, which was significantly higher compared with adjacent normal tissues (9% and 11%, respectively).Conclusions: The disordered expression of GCLC and GCLM in gastric adenocarcinoma suggested that these factors may induce tumorigenesis and may be a novel target for diagnosis and treatment of gastric adenocarcinoma.(c) 2022 Asian Surgical Association and Taiwan Robotic Surgery Association. Publishing services by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/ licenses/by-nc-nd/4.0/).