文献类型：期刊文献

英文题名：Metabolite and transcriptomic changes reveal the ripening process in Sinopodophyllum hexandrum fruit

作者：Liu, Di[1];Dong, Miaoyin[1];Li, Mengfei[1,2];Jin, Ling[3];Wei, Jianhe[4];Pare, Paul W.[5]

第一作者：Liu, Di

通信作者：Li, MF[1]

机构：[1]Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou 730070, Peoples R China;[2]Gansu Agr Univ, Coll Agron, State Key Lab Arid Land Crop Sci, Lanzhou 730070, Peoples R China;[3]Gansu Univ Tradit Chinese Med, Coll Pharm, Lanzhou 730101, Peoples R China;[4]Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China;[5]Texas Tech Univ, Dept Chem & Biochem, Lubbock, TX 79409 USA

第一机构：Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou 730070, Peoples R China

通信机构：[1]corresponding author), Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou 730070, Peoples R China.

年份：2023

卷号：206

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20234214916791);Scopus(收录号：2-s2.0-85174202908);WOS:【SCI-EXPANDED(收录号:WOS:001102126400001)】；

基金：The work was funded by the National Natural Science Foundation of China (81560617) , Assurance Project of Ecological Planting and Quality of Daodi Herbs (202103003) , and earmarked fund for CARS (CARS-21) .

语种：英文

外文关键词：Sinopodophyllum hexandrum; Fruit ripening; Podophyllotoxin; Flavonoids; Polysaccharides; Gene expression

摘要：Sinopodophyllum hexandrum is a perennial herb that produces an aryl-tetralin-type lignan, podophyllotoxin (PPT) isolated from rhizomes and used as a tumor inhibitor. Fruit from the plant, also called Himalayan Mayapple, is used as well in traditional Chinese medicine with biologically active metabolites including lignans, flavonoids, phenolics, and polysaccharides. During fruit maturation, a significant alteration in metabolite composition oc-curs. To characterize and monitor formation and turnover of metabolites during ripening, solvent-extractions of fruit were analyzed via UPLC-ESI-MS/MS during four developmental stages: young-(S1), expansion-(S2), mature-(S3) and softened-fruit (S4). To study enzymatic processes involving fruit ripening, Mayapple transcripts were monitored by RNA-seq and validated by qRT-PCR. PPT content significantly decreased while total flavonoid and polysaccharide content increased during ripening. Twenty-five differentially accumulated flavonoids (DAFs) and 1775 differentially expressed genes (DEGs) were observed during one or more of the developmental stages. Based on functional annotation, there were 116 and 82 DEGs associated with phenylpropanoid metabolism (i.e., PPT and flavonoids) and glycometabolism (e.g., starch, sucrose, and glucose), respectively. Additionally, differential expression of transcription factors (TFs) (e.g., MYB, bHLH, and WRKY) in fruits supported the phenylpropanoid metabolism and glycometabolism. Gene expression levels associated with PPT biosynthesis (e.g., DIRs, PLRs, and SDHs), flavonoids (e.g., CHSs, F3 ' Hs, and F3 ' 5 ' Hs), and polysaccharides (e.g., INV, GOLS2, and AGLU), as well as TFs (e.g., MYB1, BLH2, and WRKY65) were consistent with changing levels of metabolites detected during ripening. These findings track dynamic alterations in ripening from bitter to sweet during fruit maturation. Moreover, these results allow for the prediction of metabolite occurrence and abundance during Mayapple fruit maturation.