文献类型：期刊文献

中文题名：黄芪皂苷对大鼠萎缩性胃炎的治疗作用及对MyD88,TLR4受体的影响

英文题名：Therapeutic Effects of Astragalus Saponin on Rats with Atrophic Gastritis and MyD88,TLR4 Receptor

作者：张莲[1];周语平[1];刘光炜[1]

第一作者：张莲

机构：[1]甘肃中医学院,兰州730000

第一机构：甘肃中医药大学

年份：2013

卷号：19

期号：2

起止页码：226

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2011】；

基金：甘肃中医学院研究生创新基金项目(CX2012-05)

语种：中文

中文关键词：黄芪皂苷;慢性萎缩性胃炎;Toll样受体4;髓样分化因子88

外文关键词：astragalus saponin; CAG; TLR4; MyD88

摘要：目的:观察黄芪皂苷对萎缩性胃炎(CAG)大鼠的治疗作用,以及对髓样分化因子(MyD88),Toll样受体4(TLR4)受体的影响。方法:采用综合法造模,建立大鼠萎缩性胃炎模型,造模成功后,分别用生理盐水、黄芪皂苷(14.93 mg.kg-1)和硫糖铝(360 mg.kg-1)ig。在ig的10周、15周时分别处死大鼠,取大鼠全胃,取材、切片,HE染色,固定切片后,显微镜下观察有无肠上皮化生、假性幽门腺化生、不典型增生的发生情况,检测不同组别大鼠各期的胃组织中的MyD88,TLR4的表达,比较各组间MyD88,TLR4表达的差异,并检测血清中超氧化物歧化酶(SOD)活性,研究黄芪皂苷对萎缩性胃炎大鼠的治疗作用。结果:黄芪皂苷对萎缩性胃炎大鼠具有治疗作用,黄芪皂苷治疗后,肠上皮化生、假性幽门腺化生、不典型增生的发生情况明显减少,同时MyD88,TLR4的表达较空白组、硫糖铝组明显降低,而SOD活性的表达以黄芪皂苷组较高,与空白组相近,其次为硫糖铝组。结论:黄芪皂苷可以阻止MyD88,TLR4受体的激活,提高SOD活性,对于萎缩性胃炎具有治疗作用。
Objective： To observe the therapeutic effects of astragalus saponin on rats with atrophic gastritis （ CAG）, and on myeloid differentiation factor 88 （ MyD88）, Toll-like receptor 4 （TLR4） receptor. Method： The rat model of atrophic gastritis was established with synthesis method. Then saline, astragalus saponin （14.93 mg· kg-1） and sueralfate （360 mg·kg-1） were given orally after the modeling rats were sacrificed after 10 weeks and 15 weeks, and whole stomach was collected to observe whether there were intestinal metaplasia, pseudo-pyloric metaplasia, atypical hyperplasia under the microscope, the expression of MyD88 and TLR4 was detected. Result： Astragalus saponin had a therapeutic effect on rats with atrophic gastritis, the occurrence of intestinal metaplasia, pseudo-pyloric metaplasia, atypical hyperplasia significantly reduced, at the same time, the expression of TLR4 and MyD88 decreased significantly compared with blank group and sucralfate group, higher expression of superoxide dismutase （SOD） activity in astragalus saponin group was found. Conclusion： Astragalus saponin can prevent the activation of MyD88, TLR4 receptors and improve the activity of SOD, it has a therapeutic effect on atrophic gastritis.