文献类型：期刊文献

英文题名：Potential molecular mechanism of Guiqi Baizhu Decoction in radiation-induced intestinal edema by regulating HIF-1a, AQP4 and Na⁺/ K⁺-ATPase

作者：Li, Yangyang[1];Zhang, Liying[1,5];Zhang, Yiming[1];Miao, Zhiming[1];Liu, Zhiwei[1];Zhou, Gucheng[1];He, Jinpeng[2,3];Ding, Nan[2,3];Zhou, Heng[2,3];Zhou, Ting[1];Niu, Fan[1];Li, Jing[1];Liu, Yongqi[1,4,6]

第一作者：李燕云;李圆圆;李永玉

通信作者：Liu, YQ[1]

机构：[1]Gansu Univ Chinese Med, Prov Level Key Lab Mol Med Major Dis & Prevent & T, Lanzhou, Peoples R China;[2]Chinese Acad Sci, Key Lab Space Radiobiol Gansu Prov, Lanzhou, Peoples R China;[3]Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med Chinese Acad S, Lanzhou, Peoples R China;[4]Key Lab Med & Translat, Minist Educ Dunhuang, Lanzhou, Peoples R China;[5]Gansu Inst Cardiovasc Dis, Lanzhou, Peoples R China;[6]Gansu Univ Chinese Med, 35 Dingxi East Rd, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, 35 Dingxi East Rd, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2022

卷号：107

外文期刊名：PHYTOMEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000862466100001)】；

基金：This study was funded by the Basic Research Innovation Group (NO. 20JR10RA332). National Natural Science Foundation of China (NO. 81960869, 82004094). China Postdoctoral Science Foundation Project (NO. 2021M693794). Lanzhou City Health Key Science and Technology Development Project (NO. 2021006).

语种：英文

外文关键词：Radiation-induced intestinal edema; HIF-1 alpha; AQP4; Na+/K+-ATPase; Guiqi Baizhu decoction; Protective effect

摘要：Background: Guiqi Baizhu Decoction (GQBZD) has a good protective effect on radiation-induced intestinal edema (RIIE). However, the underlying molecular mechanisms need further elucidation. Purpose: To reveal the potential mechanism of RIIE and GQBZD treatment. Methods: SD rats were irradiated with 6Gy X-ray to establish RIIE model. The general condition of the rats was observed; the dry/wet weight ratio of colon tissue was detected; the morphological changes of colon tissue were observed by HE staining; the expressions of ROS, HIF-1 alpha and AQP4 in colon tissue were detected by confocal laser scanning; the expression of edema-related proteins was detected by Western blot. In addition, human colon epithelial cells (NCM460) was irradiated with 2Gy X-ray, and HIF-1 alpha expression in NCM460 was knocked down by small interfering RNA (siRNA) transfection, and the activity of Na+/K+-ATPase was detected by enzyme activity kit; the ROS expression was detected by flow cytometer; the AQP4 expression was detected by laser confocal microscopy; and the expression of edema-related proteins were detected by Western blot. Results: We found that after irradiation, the colon tissue of rats was significantly edema, mainly manifested as mucosal and submucosal edema, and the ultrastructure was reflected in the structural damage of nucleus and mitochondria. ROS, HIF-1 alpha and AQP4 were significantly expressed, and Na+/K+-ATPase expression/activity was decreased. After the intervention of GQBZD, the edema of the colon tissue of the rats was improved, the ex-pressions of ROS, HIF-1 alpha and AQP4 were decreased, and the expression/activity of Na+/K+-ATPase was increased. Conclusion: Ionizing radiation (IR) can cause significant intestinal edema. AQP4 and Na+/K+-ATPase are the key factors of RIIE, which are regulated by ROS and HIF-1 alpha. GQBZD can improve hypoxia and oxidative stress, regulate the expression of AQP4 and Na+/K+-ATPase, and achieve a protective effect on RIIE. This study is the first to reveal the mechanism of RIIE.