文献类型：期刊文献

英文题名：Knockdown of Microtubule Associated Serine/threonine Kinase Like Expression Inhibits Gastric Cancer Cell Growth and Induces Apoptosis by Activation of ERK1/2 and Inactivation of NF-κB Signaling

作者：An, Cai-xia[1,7];Xie, Shou-pin[2];Li, Hai-long[3];Hu, Yong-hua[3];Niu, Rong[4];Zhang, Lin-jie[5];Jiang, Yan[5];Li, Qiang[6];Zhou, Yong-ning[1]

第一作者：An, Cai-xia

通信作者：Zhou, YN[1]

机构：[1]Lanzhou Univ, Div Gastroenterol & Hepatol, Hosp 1, Lanzhou 730000, Peoples R China;[2]First Peoples Hosp Lanzhou City, Dept Neurol, Lanzhou 730050, Peoples R China;[3]Gansu Univ Chinese Med, Sch Clin Med 1, Dept Internal Mddicine, Lanzhou 730000, Peoples R China;[4]Gansu Prov Canc Hosp, Dept External Chest, Lanzhou 730030, Peoples R China;[5]Gansu Prov Maternal & Child Hlth Hosp, Div Pediat Emergency, Lanzhou 730050, Peoples R China;[6]Lanzhou Univ, Div Neurosurg, Hosp 2, Lanzhou 730000, Peoples R China;[7]Gansu Prov Matern & Child Care Hosp, Lanzhou, Peoples R China

第一机构：Lanzhou Univ, Div Gastroenterol & Hepatol, Hosp 1, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Div Gastroenterol & Hepatol, Hosp 1, Lanzhou 730000, Peoples R China.

年份：2021

卷号：41

期号：1

起止页码：108

外文期刊名：CURRENT MEDICAL SCIENCE

收录：;Scopus(收录号：2-s2.0-85100838063);WOS:【SCI-EXPANDED(收录号:WOS:000617668400016)】；

基金：This study was supported in part by grants from Lanzhou Science and Technology Planning Project (No. 2016-3-113), University Research Project of Gansu Province (No. 2018A049), the Foundation of the Fundamental Scientific Research Funds for Colleges and Universities in Gansu Province [No. (2014)63-15], the China's National Science and Technology Program for Public Wellbeing (No. 2012GS620101), and National Key Research and Development Planning (No. 2017YFC0908302).

语种：英文

外文关键词：gastric cancer; microtubule-associated serine; threonine kinase; gene expression; shRNA

摘要：Microtubule-associated serine/threonine kinase (MASTL) functions to regulate chromosome condensation and mitotic progression. Therefore, aberrant MASTL expression is commonly implicated in various human cancers. This study analyzed MASTL expression in gastric cancer vs. adjacent normal tissue for elucidating the association with clinicopathological data from patients. This work was then extended to investigate the effects of MASTL knockdown on tumor cells in vitro. The level of MASTL expression in gastric cancer tissue was assessed from the UALCAN, GEPIA, and Oncomine online databases. Lentivirus carrying MASTL or negative control shRNA was infected into gastric cancer cells. RT-qPCR, Western blotting, cell viability, cell counting, flow cytometric apoptosis and cell cycle, and colony formation assays were performed. MASTL was upregulated in gastric cancer tissue compared to the adjacent normal tissue, and the MASTL expression was associated with advanced tumor stage, Helicobacter pylori infection and histological subtypes. On the other hand, knockdown of MASTL expression significantly reduced tumor cell viability and proliferation, and arrested cell cycle at G(2)/M stage but promoted tumor cells to undergo apoptosis. At protein level, knockdown of MASTL expression enhanced levels of cleaved PARP1, cleaved caspase-3, Bax and p-ERK1/2 expression, but downregulated expression levels of BCL-2 and p-NF-kappa B-p65 protein in AGS and MGC-803 cells. MASTL overexpression in gastric cancer tissue may be associated with gastric cancer development and progression, whereas knockdown of MASTL expression reduces tumor cell proliferation and induces apoptosis. Further study will evaluate MASTL as a potential target of gastric cancer therapeutic strategy.