文献类型：期刊文献

中文题名：藏药柳茶提取物对异烟肼引起的肝损伤小鼠膜转运体Mrp2和Mrp6表达影响

英文题名：The effect of extracts Sibraea angustata on the expression of Mrp2 and Mrp6 of INH- induced hepatotoxicity in mice

作者：王汉国[1];武燕[2];岳汉萍[3]

第一作者：王汉国

机构：[1]甘肃中医药大学定西校区,甘肃定西743000;[2]甘肃中医药大学临床医学院,甘肃兰州730030;[3]甘肃省经济贸易学校,甘肃兰州730030

第一机构：甘肃中医药大学定西校区

年份：2016

卷号：27

期号：11

起止页码：2594

中文期刊名：时珍国医国药

外文期刊名：Lishizhen Medicine and Materia Medica Research

收录：北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：甘肃省自然科学基金(No.1506RJYA039)

语种：中文

中文关键词：柳茶提取物;异烟肼;肝损伤;Mrp2和Mrp6

外文关键词：Sibraea angustata ; INH ; Liver injury ; Mrp2 and Mrp6

摘要：目的研究柳茶提取物对异烟肼引起小鼠肝损伤的肝脏多药耐药相关蛋白Mrp2和Mrp6的调节作用。方法 60只小鼠随机分为模型组、柳茶提取物低、中、高剂量组、水飞蓟宾胶囊阳性对照组和空白对照组,然后各组给与相应的药物,干预结束后,采用Western Blotting技术检测并比较各组小鼠的Mrp2和Mrp6蛋白表达的变化。结果与模型组比较,经柳茶提取物干预保护后,Mrp2表达明显降低(P<0.05),而Mrp6的表达明显升高(P<0.05)。结论柳茶提取物对INH诱导的肝损伤的保护作用可能与下调肝细胞Mrp2和上调Mrp6的表达有关。
Objective To investigate the influences of the extracts of Sibraea angustata （ESA） on the expression of multidrug resistance protein 6 （Mrp6） and multidrug resistance protein 2 （Mrp2） in mice with liver damage induced by INH. Methods Totally 60 mice were randomly divided into model group, small - dosage, medium - dosage and large - dosage groups of extracts of Sibraea angustata, Silybin Capsules positive control group and control group. Each group was respectively perfused with corre-sponding drug for 10 days, then the expression of Mrp2 and Mrp6 of all mice were measured and compared. Results Compared with the model group, the expression of Mrp2 in ESA - treated groups were decreased obviously （ P 〈 0.05）, when Mrp6 were increased obviously （P 〈 0.05）. Conclusion ESA exerts its hepatoprotective activity against INH - induced hepatotoxicity of mice may be related to down regulation of Mrp2 and up regulation of Mrp6 in liver.