文献类型：期刊文献

英文题名：Changes in intestinal barrier protein expression and intestinal flora in a rat model of visceral hypersensitivity

作者：Zhao, Li[1];Ren, Peipei[2];Wang, Miaolei[2];Wang, Jingjing[1];He, Xueyun[1];Gu, Jingyan[1];Lu, Yanyu[3];Wu, Yana[2];Liu, Junhong[2];Wang, Longde[2];Li, Hongfang[1,4]

第一作者：Zhao, Li

通信作者：Li, HF[1]

机构：[1]Lanzhou Univ, Dept Physiol, Coll Basic Med, 199 Donggang West Rd, Lanzhou 730000, Peoples R China;[2]Gansu Univ Chinese Med, Affiliated Hosp, Lanzhou, Peoples R China;[3]Lanzhou Univ, Funct Lab, Coll Basic Med, Lanzhou, Gansu, Peoples R China;[4]Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou, Peoples R China

第一机构：Lanzhou Univ, Dept Physiol, Coll Basic Med, 199 Donggang West Rd, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Dept Physiol, Coll Basic Med, 199 Donggang West Rd, Lanzhou 730000, Peoples R China.

年份：2022

卷号：34

期号：4

外文期刊名：NEUROGASTROENTEROLOGY AND MOTILITY

收录：;Scopus(收录号：2-s2.0-85120653040);WOS:【SCI-EXPANDED(收录号:WOS:000722252000001)】；

基金：This work was supported by the National Natural Science Foundation of China (NSFC81660773) and (NSFC82160883).

语种：英文

外文关键词：aquaporin; intestinal flora; serine protease activity; tight junction proteins; visceral hypersensitivity

摘要：Background Destruction of the intestinal mucosal barrier and visceral hypersensitivity are main pathogenesis of irritable bowel syndrome (IBS). The study aimed to establish a rat model of visceral hypersensitivity and explore mechanisms involved the changes of the intestinal barrier protein expression and intestinal flora. Methods A rat model of visceral hypersensitivity was established and evaluated using abdominal withdrawal reflex (AWR) scores, colonic paracellular permeability, and gastrointestinal motility. The expression of tight junction proteins, aquaporin proteins (AQPs), phosphorylated ERK, and proteinase-activated receptor-2 (PAR-2) was determined. The intestinal microflora was evaluated by high-throughput sequencing of the 16S rRNA gene. Key results In model rats, AWR score and fecal water content were significantly increased, gastrointestinal motilities were disorder and characterized by an inhibition of gastric motility and an enhancement of small intestinal and colonic movement. The expressions of colonic occludin, ZO-1, AQP3, and AQP8 were decreased but claudin-2 and claudin-4 were markedly increased. Imbalance of intestinal flora appeared and showed an obvious decrease of Lactobacillus and an increase of Clostridiales_bacterium. Additionally, the total serine protease activity in feces, the expressions of PAR2 and phosphorylated ERK in the colon tissues were increased significantly. Conclusion and inferences The model rats of visceral hypersensitivity possess the decreased expression of occludin, ZO-1, AQP3, AQP8, and the increased expression of claudin-2 and claudin-4, meanwhile develop an imbalance of intestinal flora which probably increase serine protease activity, thereby activating the PAR2/ERK signaling and causing the intestinal barrier disorder.