文献类型：期刊文献

中文题名：一种固定化黄嘌呤氧化酶的制备与性能评价

英文题名：Preparation and Property Evaluation of a New Immobilized Xanthine Oxidase

作者：孙小明[1];樊瑞娜[1,2];裴栋[1];邸多隆[1];郭玫[2];赵磊[2]

第一作者：孙小明

通信作者：Di, Duo-Long

机构：[1]中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃兰州730000;[2]甘肃中医药大学药学系,甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000

第一机构：中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃兰州730000

年份：2016

卷号：33

期号：4

起止页码：396

中文期刊名：精细化工

外文期刊名：Fine Chemicals

收录：CSTPCD;;EI(收录号：20161902344559);Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家自然科学基金(21205126,21175142,21374128);国家高技术研究发展计划(863计划,2014AA022203)~~

语种：中文

中文关键词：黄嘌呤氧化酶;碳纳米管;固定化;稳定性;功能材料

外文关键词：xanthine oxidase ; carbon nanotubes ; immobilization ; stability ; iunctional matenals

摘要：为了使黄嘌呤氧化酶（XOD）可回收且能够长时间多次使用,该研究以色谱级硅球（SiO_2）为载体,采用共价键合的方式用碳纳米管（CNTs）对硅球进行包裹,并将XOD在碳纳米管包裹的硅胶球上进行固定,制备了一种以碳纳米管为介质的固定化黄嘌呤氧化酶（XOD@CNTs@SiO_2）。采用TEM和FTIR对固定化酶进行表面形貌和反应基团变化的表征;以产物尿酸为指标进行相对酶活性的评价;采用Bradford法对载体的担载量进行评价。结果表明,硅球表面经CNTs包裹后,XOD的担载量和相对酶活性分别提高2倍和2.6倍。XOD@CNTs@SiO_2在60℃储存时,储存时间至400 min后,活性仍能保持至最初的80%。4℃避光保存时,第11 d时相对活性仍为60%~70%。室温反应,连续使用至9次时,XOD@CNTs@SiO_2-30的相对酶活性仍能保留66.1%。
Abstract： In order to make the xanthine oxidase （XOD） to be recoverable and durable, a new kind of immobilized XOD was prepared. The silica microspheres were chosen as the supporter and the carbon nanotubes（CNTs） were coated on the surface of microspheres by covalent bonding. Then, free XOD were immobilized on the surface of XOD@ CNTs@ SiO2. The morphology and chemical group on the new XOD@ CNTs@ SiO2 were characterized by TEM and FTIR, respectively. Uric acid, the reaction product,was used as the evaluation indicator of the relative activity. The loading of XOD was calculated by Bradford method. It was shown that, the loading and relative activities of XOD@ CNTs@ SiO2 were increased 2 and 2.6 times than XOD@ SiO2. The relative activity could keep 80% after saving for 400 min under 60 ℃. The XOD@ CNTs@ SiO2 were kept under 4 ℃ and away from light, and the relative activity could still keep 60% ～ 70% until the 11th day. Under the normal temperature, the relative activity could keep 66. 1% after 9 times continuous reactions.