文献类型：期刊文献

中文题名：地龙降压胶囊对自发性高血压大鼠凝血相关的血小板miRNAs的影响

英文题名：Effect of Dilong antihypertension Capsule on miRNAs of Coagulation-related Platelets in Spontaneously Hypertensive Rats

作者：王碧莹[1];李应东[1];刘永琦[1,2];张小平[1,2];岳晓华[1];李长天[1];谢晓蓉[1];孙少伯[1]

第一作者：王碧莹

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃中医药大学敦煌医学与转化省部共建教育部重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2020

卷号：29

期号：1

起止页码：13

中文期刊名：中国民族民间医药

外文期刊名：Chinese Journal of Ethnomedicine and Ethnopharmacy

基金：甘肃省中医药管理局自然科学基金资助课题(GZK-2016-11);国家自然科学基金资助课题(81360345)

语种：中文

中文关键词：地龙降压胶囊;miRNAs;凝血相关

外文关键词：Dilong antihypertension Capsule;miRNAs;Coagulation-related

摘要：目的:通过地龙降压胶囊对SHR大鼠凝血相关的血小板miRNAs表达的影响,探讨地龙降压胶囊的降压机制。方法:SHR大鼠分为5组:正常对照组、肝素阳性对照组、地龙降压胶囊低、中、高剂量组。药物高、中、低组分别灌胃地龙降压胶囊100 mg/kg、50 mg/kg、25 mg/kg。15 d后取血,实时荧光定量PCR(RT-PCR)方法检测5种miRNAs的表达水平。结果:与正常对照组相比,地龙降压胶囊干预组与血小板活化相关的miRNA-223、miRNA-96和聚集相关的miRNA-130a的表达量明显改变,而与血小板生成有关的miRNA-155、miRNA34a-5p的表达量无明显变化。结论:地龙降压胶囊可能主要通过影响血小板活化与聚集相关的miRNAs发挥抗凝血降压作用。
Objective To explore the hypotensive mechanism of Dilong antihypertension Capsule, based on the investigations of the expressions of miRNAs coagulation-related platelets after the administration of Dilong antihypertension Capsule. Methods SHR rats were randomly divided into 5 groups: normal control group, heparin-positive control group, low-dose group of Dilong antihypertension Capsule, medium-dose group of Dilong antihypertension Capsule, high-dose group of Dilong antihypertension Capsule. The high, medium and low-dose groups were respectively given Dilong antihypertension Capsule 100 mg/kg, 50 mg/kg and 25 mg/kg. Bloods were collected 15 days after administration, and the expressions of five miRNAs were detected by real-time fluorescence quantitative PCR(RT-PCR). Results Compared with the control group, the expressions of platelet activation-related miRNA-223, miRNA-96 and conglomeration-related miRNA-130 a in Dilong antihypertension Capsule-treated groups changed significantly, while there was no obvious change in the expressions of miRNA-155 and miRNA34 a-5 p related to thrombocytopoiesis. Conclusion Dilong antihypertension Capsule may play an anticoagulant and hypotensive role mainly by affecting the miRNAs expressions of platelet activation and aggregation.