文献类型：期刊文献

中文题名：参桂保心方对慢性心力衰竭大鼠基质金属蛋白酶2、基质金属蛋白酶抑制剂1、B型脑钠肽及心室重构的影响

英文题名：Effects of ShenGuiBaoXin Decoction on Matrix Metalloproteinase-2,the Tissue Inhibitor of Matrix Metalloproteinase-1,Brain Natriuretic Peptide,and Ventricular Remodeling in Rats with Chronic Heart Failure

作者：卢玉俊[1];赵信科[1];代晶晶[1]

第一作者：卢玉俊

机构：[1]甘肃中医药大学附属医院心血管临床医学中心,兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2018

卷号：47

期号：3

起止页码：231

中文期刊名：中国医科大学学报

外文期刊名：Journal of China Medical University

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2017_2018】；

基金：甘肃省自然科学基金(148RJZA068)

语种：中文

中文关键词：慢性心力衰竭;基质金属蛋白酶2;基质金属蛋白酶抑制剂1;B型脑钠肽;参桂保心方

外文关键词：chronic heart failure; matrix metalloproteinases-2; tissue inhibitor of matrix metalloproteinase-1 ; brain natriuretic peptide ;ShenGuiBaoXin decoction

摘要：目的观察参桂保心煎剂对心力衰竭大鼠基质金属蛋白酶2(MMP-2)、基质金属蛋白酶抑制剂1(TIMP-1)、B型脑钠肽(BNP)水平及其对心肌组织的影响,探讨该药对心室重构的抑制作用。方法将90只SD大鼠分为2组,对照组15只,注射盐水;实验组75只,注射异丙肾上腺素3 mg/(kg·d),4周后射血分数≤50%为造模成功。将实验组大鼠分为模型组,芪苈强心组,参桂保心高、中、低剂量组,每组15只。8周后测定心胸比,BNP,心肌MMP-2、TIMP-1表达,并进行HE染色。结果心胸比和BNP在治疗后均有下降,芪苈强心组及高剂量组尤为明显;MMP-2表达在治疗后均有减少,其中,高剂量组优于芪苈强心组。结论参桂保心可能通过减少MMP-2表达,调节MMP-2/TIMP-1的动态平衡,维持细胞外基质合成与降解平衡,达到抑制心室重构的作用。
Objective To observe the effects of Shen Gui Bao Xin decoction on matrix metalloproteinases-2（MMP-2）,the tissue inhibitor of matrix metalloproteinase-1（TIMP-1）,brain natriuretic peptide（BNP） levels,and cardiac histopathology in rats with heart failure,and to investigate the mechanism of the inhibition effect on ventricular remodeling in rats with heart failure. Methods Ninety male SD rats were randomly divided into two groups,with 15 rats in the control group and 75 rats in the model group. Normal saline was injected in the normal control group. The model group was given an intraperitoneal injection of isoprenaline 3 mg/（kg·d）. After 4 weeks,an ejection fraction of ≤50% on echocardiography was considered a successful model. Seventy-five rats were randomly divided into the model,Qiliqiangxin,and Shen Gui Bao Xin groups（high,medium,and low doses）,with 15 rats in each group. Eight weeks later,the cardiothoracic ratio and BNP level were measured,hematoxylin-eosin staining was performed,and the MMP-2 and TIMP-1 protein expressions in the myocardium was detected. Results In terms of cardiothoracic ratio and BNP level,all the groups showed decreases after treatment,especially the Qiliqiangxin and high-dose groups. The MMP-2 protein expression level was decreased in all the groups after the treatment,and the high-dose group was superior to the Qiliqiangxin group. Conclusion Shen Gui Bao Xin decoction functions by reducing the MMP-2 expression level,regulating MMP-2/TIMP-1 dynamic balance,maintaining the balance of synthesis and degradation of the extracellular matrix,inhibiting ventricular remodeling,and improving cardiac function.