文献类型：期刊文献

中文题名：芍药汤对大肠湿热型溃疡性结肠炎大鼠结肠组织中CD14,FADD,Caspase-8表达的影响

英文题名：Effect of Shaoyaotang on Expressions of CD14, FADD and Caspase-8 in Colonic Tissues of Rats with Large Intestinal Damp-heat Syndrome of Ulcerative Colitis

作者：曹思齐[1];王凤仪[1,2];汤胜男[1];赵党生[1,2];李洋洋[1];刘志杰[1];柴瑞婷[1]

第一作者：曹思齐

机构：[1]甘肃中医药大学,兰州730000;[2]深圳市宝安纯中医治疗医院,广州深圳518101

第一机构：甘肃中医药大学

年份：2021

卷号：27

期号：5

起止页码：1

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金项目(8156150228)。

语种：中文

中文关键词：芍药汤;溃疡性结肠炎;白细胞分化抗原14(CD14);Fas相关死亡结构域蛋白(FADD);天冬半胱氨酸蛋白酶-8(Caspase-8)

外文关键词：Shaoyaotang;ulcerative colitis;leukocyte differentiation antigen 14(CD14);Fas-related death domain protein(FADD);cysteinyl aspartate specific protease-8(Caspase-8)

摘要：目的:观察芍药汤对大肠湿热型溃疡性结肠炎(UC)模型大鼠血清中细胞黏附分子-1(ICAM-1),转化生长因子-β1(TGF-β1)含量及病灶结肠组织中白细胞分化抗原14(CD14),Fas相关死亡结构域蛋白(FADD)与天冬半胱氨酸蛋白酶-8(Caspase-8)mRNA及蛋白表达的影响。方法:将SPF级Wistar大鼠80只随机分为正常组10只、造模组70只,采用病证结合的方式复制大肠湿热型UC大鼠,即高脂高糖辛辣饮食+2,4-二硝基苯磺酸(DNBS)结合乙醇复合法造模,造模成功后,按随机数字表法将造模组分为模型组、柳氮磺砒啶(SASP)组及芍药汤低、中、高剂量组,每组14只,给予柳氮磺嘧啶0.2 g·kg^(-1)·d^(-1),芍药汤低、中、高剂量(6,12,24 g·kg^(-1)·d^(-1))灌胃,正常组及模型组给予等体积生理盐水灌胃,连续21 d。采用酶联免疫吸附测定(ELISA)法检测血清ICAM-1,TGF-β1含量,实时荧光定量聚合酶链式反应(Real-time PCR)检测结肠组织CD14,FADD,Caspase-8 mRNA表达,蛋白免疫印迹法(Western blot)检测结肠组织CD14,FADD,Caspase-8蛋白表达。结果:与正常组比较,模型组大鼠血清中ICAM-1含量明显升高,TGF-β1含量明显降低(P<0.05);CD14,FADD,Caspase-8 mRNA及蛋白相对表达量明显升高(P<0.05)。与模型组比较,芍药汤中、高剂量组及SASP组大鼠血清中ICAM-1含量明显降低,而芍药汤低、中、高剂量组及SASP组大鼠血清中TGF-β1含量明显升高(P<0.05);各干预组CD14,FADD,Caspase-8 mRNA及蛋白表达量明显降低(P<0.05),并以芍药汤高剂量组及SASP组更为明显。结论:芍药汤对大肠湿热型UC大鼠具有一定干预作用,其机制可能与抑制CD14,FADD及Caspase-8 mRNA及蛋白的表达有关。
Objective:To observe the effect of Shaoyaotang on the contents of cell adhesion molecule-1(ICAM-1)and transforming growth factor-β1(TGF-β1)in serum of large intestine damp-heat syndrome of ulcerative colitis(UC)in rats,and the gene and protein expressions of leukocyte differentiation antigen14(CD14),Fas-related death domain protein(FADD)and cysteinyl aspartate specific protease-8(Caspase-8)in the focal colon tissue. Method: A total of 80 SPF Wistar rats were randomly divided into the blank group(n=10)and modeling group(n=70). The large intestine damp-heat syndrome of UC rats was replicated by the combination of disease and syndrome,which was high-fat,high-sugar and spicy diets combined with 2,4-dinitrobenzene sulfonic acid(DNBS)and ethanol. After successful modeling,the modeled groups were divided into model group,sulfasalazine(SASP)control group,and low,medium and high-dose Shaoyaotang groups by the method of random number table,with14 rats in each group. Low,medium and high doses of Sulfasalazine0.2 g·kg^(-1)·d^(-1) and Shaoyaotang(6,12,24 g·kg^(-1)·d^(-1))were given by gavage. The blank group and the model group were given equal volume of normal saline for 21 days. The contents of serum ICAM-1 and TGF-β1 were detected by enzyme-linked immunosorbent assay(ELISA),the expressions of CD14,FADD and Caspase-8 mRNA in colon tissues were detected by Real-time quantitative polymerase chain reaction(Real-time PCR),and the expressions of CD14,FADD and Caspase-8 protein in colon tissues were detected by Western blot.Result: Compared with the blank group,the serum ICAM-1 level in the model group were significantly increased,whereas the content of TGF-β1 were significantly decreased(P<0.05). The relative expression levels of CD14,FADD,Caspase-8 mRNA and protein were significantly increased(P<0.05). Compared with the model group,the content of ICAM-1 in the serum of the rats in the medium,high-dose Shaoyaotang groups and the SASP group were significantly decreased,while the content of TGF-β1 in the serum of the rats in the low,medium, high-dose Shaoyaotang groups and the SASP group were significantly increased(P<0.05). The expression levels of CD14,FADD,Caspase-8 mRNA and protein in each intervention group were significantly decreased(P<0.05), especially in the high-dose Shaoyaotang group and the SASP group. Conclusion:Shaoyaotang has a certain intervention effect on UC rats with large intestine damp-heat syndrome,and its mechanism may be related to the inhibition of CD14,FADD and Caspase-8 genes and proteins expression.