详细信息
当归注射液、黄芪注射液不同配比促鸡胚绒毛尿囊膜模型血管生成的实验研究 被引量:2
Study of the Different Ratio of Angelica sinensis and Astragalus membranaceus injection on Angiogenesis of Chick Embryo Chorioallantoic Membrane Model
文献类型:期刊文献
中文题名:当归注射液、黄芪注射液不同配比促鸡胚绒毛尿囊膜模型血管生成的实验研究
英文题名:Study of the Different Ratio of Angelica sinensis and Astragalus membranaceus injection on Angiogenesis of Chick Embryo Chorioallantoic Membrane Model
作者:杨丽霞[1];卢雯玲[1];牛洁[1];王志程[1];刘铜华[1];魏道武[2]
第一作者:杨丽霞
机构:[1]北京中医药大学,北京100029;[2]甘肃中医学院,甘肃兰州730000
第一机构:北京中医药大学,北京100029
年份:2008
卷号:14
期号:9
起止页码:51
中文期刊名:中国实验方剂学杂志
外文期刊名:Chinese Journal of Experimental Traditional Medical Formulae
语种:中文
中文关键词:血管生成;鸡胚绒毛尿囊膜;当归补血汤;当归注射液;黄芪注射液
外文关键词:angiogenesis; chick embryo chorioallantoic membrane; Danggui Buxue Decoction; Angelica sinensis injection; Astragalus membranaceus Injection;
摘要:目的:探讨当归、黄芪注射液不同配比对鸡胚绒毛尿囊膜(chorioallantoic membrane,CAM)血管生成的影响。方法:种蛋在37.8℃孵育7 d后制备CAM模型,当归、黄芪注射液不同配比滴加于CAM表面的载体上,继续孵育3 d,观察鸡胚存活状况,然后制备鸡胚CAM标本,解剖显微镜下计数新生毛细血管数目。结果:当归、黄芪注射液不同配比均具有促进鸡胚CAM血管生成作用,与生理盐水对照组相比有显著性差异(P<0.05),除当归黄芪1∶5,其他各组药效低于表皮生长因子(EGF)(P<0.05)。结论:当归、黄芪注射液不同配比具有显著促进鸡胚CAM血管生成作用,其可能的机制是血管内皮细胞的增殖与迁移。
Objective: To investigate the effect of the different ratio of Angelica sinensis Injection(ASI) and Astragalus membranaceus Injection(AMI) on angiogenesis of the chick embryo chorioallantoic membrane(CAM).Methods: After fertilized eggs were incubated for 7 days at(37.8 ℃,) the chorioallantoic membrane(CAM)models were made,and were respectively treated with NS(negative control),different ratio of ASI and AMI,and EGF(positive control).After incubated for another 3 days,the CAM samples were made,then counted t...
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