文献类型：期刊文献

中文题名：当归多糖抗运动疲劳作用及其机制研究

英文题名：Study on Anti-fatigue Effect and Mechanism of Angelica sinensis Polysaccharide

作者：黄彩云[1];何吉福[1];宋春虹[1];王楠[1];马玉德[1]

第一作者：黄彩云

机构：[1]甘肃中医药大学体育健康学院,甘肃兰州730000

第一机构：甘肃中医药大学体育健康学院

年份：2022

卷号：32

期号：5

起止页码：434

中文期刊名：康复学报

外文期刊名：Rehabilitation Medicine

收录：CSTPCD;;CSCD:【CSCD_E2021_2022】；

基金：甘肃省自然科学基金项目(1606RJZA197);国家自然科学基金项目(82160755);甘肃中医药大学科技创新项目(30740301);甘肃省教育厅高等学校科研能力提升项目(2021B-157)。

语种：中文

中文关键词：当归多糖;抗疲劳;乳酸;尿素氮;肝糖原;肌糖原;ATP酶

外文关键词：Angelica sinensis polysaccharide;anti-fatigue;lactic acid;urea nitrogen;liver glycogen;muscle glycogen;ATPase

摘要：目的:探讨不同剂量当归多糖(ASP)对小鼠的抗疲劳作用及其机制。方法:选择SPF级雄性昆明小鼠50只,按照随机数字表法分为空白对照组、模型组、ASP高剂量组、ASP中剂量组和ASP低剂量组,每组10只。除空白对照组外,各组小鼠负重游泳至力竭1次/d,持续负重游泳15 d。造模第6天开始,ASP高、中、低剂量组小鼠分别灌胃相应剂量(222.56、111.28、55.64 mg/kg)的药物,空白对照组和模型组仅以等量生理盐水灌胃,连续灌胃10 d。记录最后1次小鼠负重游泳至力竭的时间,评估小鼠运动耐力;而后采集标本,检测血清肌糖原、肝糖原、血乳酸及血尿素氮含量;检测肝脏、肾脏及后肢肌肉中的ATP酶含量。结果:①与模型组比较,ASP中、高剂量组小鼠负重游泳时间均有所延长(P<0.05);与低剂量组和中剂量组分别比较,高剂量组小鼠的负重游泳时间均更长(P<0.05)。②与空白对照组比较,模型组小鼠血清肌糖原和肝糖原含量均降低(P<0.05);与模型组比较,ASP中剂量组小鼠血清肝糖原含量升高(P<0.05),ASP高剂量组小鼠血清肌糖原和肝糖原含量均升高(P<0.05),其中以ASP高剂量组小鼠血清肌糖原升高最明显(P<0.05)。③与空白对照组比较,模型组小鼠血清血乳酸和尿素氮含量均升高(P<0.05);与模型组比较,ASP低剂量组小鼠血清血乳酸含量降低(P<0.05),ASP中、高剂量组小鼠血清血乳酸和血尿素氮含量均降低(P<0.05),其中以ASP高剂量组小鼠血清血乳酸降低最明显(P<0.05)。④与空白对照组比较,模型组小鼠肝脏中Na^(+)-K^(+)-ATP和Ca^(2+)-Mg^(2+)-ATP酶含量降低(P<0.05),肾脏中Na^(+)-K^(+)-ATP酶、Mg^(2+)-ATP酶和Ca^(2+)-Mg^(2+)-ATP酶含量均降低(P<0.05),后肢肌肉中的Na^(+)-K^(+)、Mg^(2+)、Ca^(2+)、Ca^(2+)-Mg^(2+)4种ATP酶含量均降低(P<0.05)。与模型组比较,ASP低剂量组小鼠后肢肌肉中Ca^(2+)-Mg^(2+)-ATP酶含量升高(P<0.05);ASP中剂量组小鼠肝脏中的Ca^(2+)-Mg^(2+)-ATP含量升高(P<0.05),后肢肌肉Mg^(2+)-ATP酶、Ca^(2+)-ATP酶和Ca^(2+)-Mg^(2+)-ATP酶含量均升高(P<0.05);ASP高剂量组小鼠肝脏中的Na^(+)-K^(+)-ATP和Ca^(2+)-Mg^(2+)-ATP酶含量均升高(P<0.05),肾脏中Na^(+)-K^(+)-ATP酶和Mg^(2+)-ATP酶含量均升高(P<0.05),后肢肌肉中Na^(+)-K^(+)、Mg^(2+)、Ca^(2+)、Ca^(2+)-Mg^(2+)4种ATP酶含量均升高(P<0.05);其中以ASP高剂量组小鼠肝脏Ca^(2+)-Mg^(2+)-ATP酶升高最明显(P<0.05)。结论:ASP在一定范围内可显著延长小鼠负重游泳时间,发挥抗疲劳功效,其作用机制与ASP可显著提高小鼠运动耐力、提升糖原储备、减少乳酸与尿素氮堆积、改善机体能量代谢水平有关。
Objective:To investigate the anti-fatigue effect and mechanism of Angelica sinensis polysaccharide(ASP)at di fferent doses in mice.Methods:A total of 50 SPF male Kunming mice were randomly divided into blank control group,model group,ASP high-dose group,ASP medium-dose group,and ASP low-dose group according to the random number table method,with 10 mice in each group.Except for the blank control group,mice in each group carried out loaded swimming to exhaustion once a day for 15 days.From the 6th day of modeling,mice in the ASP high-dose group,medium-dose group and low-dose group were in-tragastrically administered with corresponding doses of ASP(222.56,111.28,55.64 mg/kg),respectively,while mice in the blank control group and model group were given the same amount of normal saline by intragastric administration for 10 days.The time taken for loaded swimming to exhaustion in the last day was recorded,and the exercise endurance was evaluated.Then the specimens were gathered,the serum muscle glycogen,liver glycogen,blood lactic acid and blood urea nitrogen were detected,and the content of ATPase in liver,kidney and hindlimb muscle was detected.Results:1)Compared with the model group,the swimming time of mice in the ASP medium-dose group and ASP high-dose group was prolonged(P<0.05);compared with the ASP low-dose group and ASP medium-dose group,the ASP high-dose group showed longer loaded swimming time(P<0.05).2)Compared with the blank control group,the serum muscle glycogen and liver glycogen content of mice in the model group decreased(P<0.05);compared with the model group,the serum liver glycogen content of mice in the ASP medium-dose group increased(P<0.05),and the serum muscle glycogen and liver glycogen content of mice in the ASP high-dose group increased(P<0.05),and the serum muscle glycogen content of mice in the ASP high-dose group had the most significant increase(P<0.05).3)Compared with the blank control group,the serum blood lactic acid and urea nitrogen content of mice in the model group increased(P<0.05);compared with the model group,the serum blood lactate content of mice in the ASP low-dose group decreased(P<0.05),and the serum blood lactate and blood urea nitrogen content of mice in the ASP medium-dose group and ASP high-dose group decreased(P<0.05),with the most sig-nificant decrease shown in the serum blood lactic acid content of mice in the ASP high-dose group(P<0.05).4)Compared with the blank control group,the Na^(+)-K^(+)-ATPase and Ca^(2+)-Mg^(2+)-ATPase in liver of mice in the model group decreased(P<0.05),the Na^(+)-K^(+)-ATPase,Mg^(2+)-ATPase and Ca^(2+)-Mg^(2+)-ATPase in kidney decreased(P<0.05),and the Na^(+)-K^(+),Mg^(2+),Ca^(2+)and Ca^(2+)-Mg^(2+)ATPase in hindlimb muscles of mice in the model group decreased(P<0.05).Compared with the model group,the Ca^(2+)-Mg^(2+)-ATPase in hindlimb muscles of mice in the ASP low-dose group increased(P<0.05).The Ca^(2+)-Mg^(2+)-ATP in liver of the mice in the ASP medium dose group significantly increased(P<0.05),the Mg^(2+)-ATPase,Ca^(2+)-ATPase and Ca^(2+)-Mg^(2+)-ATPase in hindlimb muscle significantly increased(P<0.05),the Na^(+)-K^(+)-ATP and Ca^(2+)-Mg^(2+)-ATPase in liver significantly increased(P<0.05),and the Na^(+)-K^(+)-ATPase and Mg^(2+)-ATPase in kidney significantly increased(P<0.05).The Na^(+)-K^(+),Mg^(2+),Ca^(2+)and Ca^(2+)-Mg^(2+)ATPase in hindlimb muscle increased(P<0.05).The increase of liver Ca^(2+)-Mg^(2+)ATPase of the mice in the ASP high-dose group was the most obvious(P<0.05).Conclu-sion:ASP can significantly prolong the loaded swimming time of mice to a certain extent and play an anti-fatigue effect.The under-lying mechanism is related to the fact that ASP can significantly improve the exercise endurance,increase glycogen reserve,reduce the accumulation of lactic acid and urea nitrogen,and improve energy metabolism of mice.