文献类型：期刊文献

中文题名：胎牛血清和新生牛血清体外培养动物细胞的效果比较

英文题名：Effect Comparison of Fetal Bovine Serum and Newborn Bovine Serum for Animal Cell Culture in vitro

作者：马桂兰[1,2];暴艳敏[2];徐水林[2];孔来信[3];王家敏[1];马忠仁[1];乔自林[1]

第一作者：马桂兰

机构：[1]甘肃省动物细胞工程技术研究中心,甘肃兰州730030;[2]兰州民海生物工程有限公司,甘肃兰州730030;[3]甘肃中医学院公共课部,甘肃兰州730000

第一机构：甘肃省动物细胞工程技术研究中心,甘肃兰州730030

年份：2014

期号：2

起止页码：65

中文期刊名：黑龙江农业科学

外文期刊名：Heilongjiang Agricultural Sciences

基金：西北民族大学中央高校基本科研业务费专项资金资助项目(31920130066);甘肃省科技重大专项资助项目(1203FKDA030);国家科技基础平台资助项目(2005 DKA21101-29)

语种：中文

中文关键词：胎牛血清;新生牛血清;贴壁培养;生长曲线;集落形成率

外文关键词：fetal bovine serum;newborn bovine serum;adherent cell culture;growth curve;colony formation rate

摘要：为了比较进口胎牛血清、国产胎牛血清和国产新生牛血清的细胞培养效果,以2批进口胎牛血清、3批国产胎牛血清和2批国产新生牛血清为试验材料,采用细胞贴壁传代培养、最大增殖浓度和倍增时间以及集落形成率3种方法,比较了7批供试血清对VERO、CHO-K1、MDCK和Hela 4种细胞的培养效果。结果表明:连续传代培养时所有牛血清均有较好的促细胞生长的效果;进口胎牛血清、国产胎牛血清和国产新牛血清对4种细胞的平均最大增殖密度为67.8×104、62.1×104和64.8×104 cfu·mL-1,平均倍增时间为22.2、22.5和22.7h;对VERO、CHO-K1和Hela细胞的平均集落形成率为59.2%、57.6%和50.9%。3种牛血清对群体性细胞培养时效果差别不明显,对单细胞克隆培养时胎牛血清优于新生牛血清。
In order to compare the culture effectiveness of imported ,domestic and domestic newborn bovine ser-um for animal cells in vitro ,taking 2 batches of imported fetal bovine serum ,3 batches of domestic fetal bovine serum and 2 batches of domestic newborn bovine serum as materials ,adherent cell subculture ,low cell density growth curve and colony efficiency three methods were used to compare the cells culture effect of four cells of VERO ,CHO-K1 ,MDCK and Hela .The results showed that all bovine serum had better cell growth promoting effect by continuous subculture ;average maximum density of the imported fetal bovine serum ,fetal bovine ser-um and domestic new domestic bovine serum were 67 .8 × 104 ,62 .1 × 104 and 64 .8 × 104 cfu·mL-1 respectively , average doubling time were 22 .2 ,22 .5 and 22 .7 h;the average colony formation rate of VERO ,CHO-K1 and Hela cells were 59 .2% ,57 .6% and 50 .9% .The effect of three types of bovine serum on different groups of cells in vitro were not obvious ,the individual cells clone culture fetal bovine serum were better than newborn calf serum .