文献类型：期刊文献

英文题名：Direct multiple shoot induction and plant regeneration from dormant buds of Codonopsis pilosula (Franch.) Nannf.

作者：Zhang, Y. H.[1,2];Gao, S. F.[2];Du, T.[2];Chen, H. G.[2];Wang, H. Z.[2];Zhu, T. T.[2];Zhang, J. W.[1]

第一作者：张延红;Zhang, Y. H.

通信作者：Zhang, JW[1]

机构：[1]Gansu Agr Univ, Gansu Prov Key Lab Aridland Crop Sci, Lanzhou 730020, Gansu, Peoples R China;[2]Gansu Coll Tradit Chinese Med, Lanzhou 730000, Gansu, Peoples R China

第一机构：Gansu Agr Univ, Gansu Prov Key Lab Aridland Crop Sci, Lanzhou 730020, Gansu, Peoples R China

通信机构：[1]corresponding author), Gansu Agr Univ, Gansu Prov Key Lab Aridland Crop Sci, Lanzhou 730020, Gansu, Peoples R China.

年份：2011

卷号：10

期号：51

起止页码：10509

外文期刊名：AFRICAN JOURNAL OF BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000298391500025)】；

语种：英文

外文关键词：Codonopsis pilosula; dormant bud; direct organogenesis; multiple shoot; shoot elongation; rooting

摘要：An efficient and reproducible protocol for in vitro plant multiplication system via direct organogenesis from dormant buds of Codonopsis pilosula Nannf was developed. Multiple shoots were induced at a frequency of 75% after nine weeks on Murashige and Skoog (MS) medium supplemented with BAP (1.0 mg/l), NAA (0.5 mg/l), 3% sucrose and 0.7% agar. Approximately, 15 to 18 shoots were formed at the base of each dormant bud. Higher concentrations of BAP and NAA resulted in callus formation. Further development of shoot elongation and multiplication were also studied. Well-grown shoots of 2.8 cm height and 3.8 proliferation coefficient were achieved by sub-culturing on MS medium supplemented with 0.2 mg/l BAP and 0.05 mg/l IBA. At higher concentrations, BAP (0.5 mg/l) promoted higher shoot proliferation coefficient (4.2); however, it negatively affected shoot elongation. Further, low NAA concentration was beneficial to shoot proliferation. All in vitro-derived shoots measuring 2.5 to 3 cm in length, rooted when grown on 1/2MS (half of all MS elements) basal medium containing 1.5 mg/l IBA within 3 weeks, 100% of shoots developed roots and test-tube seedlings grew stout.