文献类型：期刊文献

英文题名：Codonopsis pilosula water extract delays D-galactose-induced aging of the brain in mice by activating autophagy and regulating metabolism

作者：Wang, Xuewen[1];Kang, Jiachao[1];Li, Xuechan[1];Wu, Pingmin[2];Huang, Yong[3];Duan, Yongqiang[4];Feng, Juan[5];Wang, Jing[1]

第一作者：王雄伟

通信作者：Wang, J[1];Feng, J[2]

机构：[1]Gansu Univ Chinese Med, Sch Publ Hlth, 35 Dingxi East Rd, Lanzhou 730000, Peoples R China;[2]Gansu Univ Chinese Med, Teaching Expt Training Ctr, Lanzhou 730000, Peoples R China;[3]Gansu Univ Chinese Med, Sch Basic Med, Lanzhou 730000, Peoples R China;[4]Ningxia Med Univ, Coll Tradit Chinese Med, Yinchuan 750004, Peoples R China;[5]Shenzhen Technol Univ, Coll Hlth Sci & Environm Engn, 3002 Lantian Rd, Shenzhen 518118, Peoples R China

第一机构：甘肃中医药大学公共卫生学院

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Publ Hlth, 35 Dingxi East Rd, Lanzhou 730000, Peoples R China;[2]corresponding author), Shenzhen Technol Univ, Coll Hlth Sci & Environm Engn, 3002 Lantian Rd, Shenzhen 518118, Peoples R China.|[10735e9d5e7087247e71b]甘肃中医药大学公共卫生学院;[10735]甘肃中医药大学;

年份：2024

卷号：327

外文期刊名：JOURNAL OF ETHNOPHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-85187992741);WOS:【SCI-EXPANDED(收录号:WOS:001209103300001)】；

基金：Funding statement This work was supported by the National Natural Science Foundation of China (No. 82060829, No. 82204756) , the Gansu Provincial Natural Science Foundation (23JRRA1720) , and the Shenzhen Science and Technology Program (No. RCBS20210609103650047) .

语种：英文

外文关键词：Codonopsis pilosula; Aging; D -galactose; Hippocampus; Autophagy; Target metabolomics

摘要：Ethnopharmacological relevance: Codonopsis pilosula (C. pilosula), also called "Dangshen" in Chinese, is derived from the roots of Codonopsis pilosula (Franch.) Nannf. (C. pilosula), Codonopsis pilosula var. Modesta (Nannf.) L.D. Shen (C. pilosula var. modesta) or Codonopsis pilosula subsp. Tangshen (Oliv.) D.Y.Hong (C. pilosula subsp. tangshen), is a well-known traditional Chinese medicine. It has been regularly used for anti-aging, strengthening the spleen and tonifying the lungs, regulating blood sugar, lowering blood pressure, strengthening the body's immune system, etc. However, the mechanism, by which, C. pilosula exerts its therapeutic effects on brain aging remains unclear. Aim of the study: This study aimed to investigate the underlying mechanisms of the protective effects of C. pilosula water extract (CPWE) on the hippocampal tissue of D-galactose-induced aging mice. Materials and methods: In this research, plant taxonomy has been confirmed in the "The Plant List" database (www.theplantlist.org). First, an aging mouse model was established through the intraperitoneal injections of Dgalactose solution, and low-, medium-, and high-dose CPWE were administered to mice by gavage for 42 days. Then, the learning and memory abilities of the mice were examined using the Morris water maze tests and stepdown test. Hematoxylin and eosin staining was performed to visualize histopathological damage in the hippocampus. A transmission electron microscope was used to observe the ultrastructure of hippocampal neurons. Immunohistochemical staining was performed to examine the expression of glial fibrillary acidic protein (GFAP), the marker protein of astrocyte activation, and autophagy-related proteins, including microtubule-associated protein light chain 3 (LC3) and sequestosome 1 (SQSTM1)/p62, in the hippocampal tissues of mice. Moreover, targeted metabolomic analysis was performed to assess the changes in polar metabolites and short-chain fatty acids in the hippocampus. Results: First, CPWE alleviated cognitive impairment and ameliorated hippocampal tissue damage in aging mice. Furthermore, CPWE markedly alleviated mitochondrial damage, restored the number of autophagosomes, and activated autophagy in the hippocampal tissue of aging mice by increasing the expression of LC3 protein and reducing the expression of p62 protein. Meanwhile, the expression levels of the brain injury marker protein GFAP decreased. Moreover, quantitative targeted metabolomic analysis revealed that CPWE intervention reversed the abnormal levels of L-asparagine, L-glutamic acid, L-glutamine, serotonin hydrochloride, succinic acid, and acetic acid in the hippocampal tissue of aging mice. CPWE also significantly regulated pathways associated with Dglutamine and D-glutamate metabolism, nitrogen metabolism, arginine biosynthesis, alanine, aspartate, and glutamate metabolisms, and aminoacyl-tRNA biosynthesis.