文献类型：期刊文献

中文题名：平痫颗粒对戊四唑点燃癫痫模型大鼠海马区凋亡调控基因蛋白表达的影响

英文题名：Effect of Pingxian granules on protein expression of apoptosis regulatory genes in hippocampus of pentylenetetrazol-induced epileptic model rats

作者：田茸[1,2];舍雅莉[1];贾育新[1];王宇[1];程小丽[1];慕宝龙[1]

第一作者：田茸

机构：[1]甘肃中医学院;[2]上海中医药大学

第一机构：甘肃中医药大学

年份：2012

期号：9

起止页码：1307

中文期刊名：中国中药杂志

外文期刊名：China Journal of Chinese Materia Medica

收录：MEDLINE(收录号：22803381);CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；PubMed;

基金：甘肃中医学院中青年基金项目(09ZQ-12)

语种：中文

中文关键词：平痫颗粒;癫痫;PTZ点燃型癫痫模型;Bcl-2;Bax

外文关键词：Pingxian granules; epilepsy; PTZ kindling epileptic rat model; Bcl-2; Bax

摘要：目的:观察平痫颗粒在阻抗癫痫形成中对大鼠海马区凋亡调控基因Bcl-2,Bax蛋白表达的影响,探讨其抗癫痫作用的分子生物学机制。方法:选择60只45 d龄Wistar大鼠,随机分为正常对照组、模型组、阳性对照组、平痫低剂量组、平痫高剂量组,每组12只。除正常对照组,其余各组大鼠均以戊四唑亚惊厥剂量35 mg.kg-1腹腔注射诱导癫痫模型。造模同时平痫高、低剂量组分别以1.66,0.42 g.mL-1的生药含量2 mL.d-1灌胃;阳性对照组给予等量3.6 g.L-1的苯巴比妥混悬液灌胃;模型组和正常对照组胃饲等量双蒸水,持续5周。采用免疫组化染色(SABC法)于3,5周末分别测定Bcl-2,Bax免疫阳性细胞。结果:①给大鼠连续注射戊四唑过程中,模型组大鼠癫痫发作级别随点燃周次增加逐渐升级,由第1周的Ⅰ～Ⅱ级至第4周时的Ⅵ级大发作。治疗组发作次数和级别相对明显降低,最高级别Ⅳ级,且组间发作级别、次数比较无显著性差异。②随着点燃周次的增加,在3,5周末Bcl-2表达模型组明显低于正常对照组(P<0.01),Bax表达模型组明显高于正常对照组(P<0.01);阳性对照组、平痫高、低剂量组Bcl-2表达均明显高于模型组(P<0.01),Bax表达阳性对照组、平痫高、低剂量组与模型组比较有明显降低(P<0.01)。结论:平痫颗粒可能是通过促进抗凋亡调控基因Bcl-2蛋白的表达,以及抑制促凋亡调控基因Bax蛋白的表达,从而减少神经元细胞的凋亡来阻抗癫痫。
Objective： To observe the effect of Pingxian granules on the protein expression of apoptosis regulatory genes Bcl-2 and Bax in the hippocampus of epileptic model rats and study the molecular biological mechanism of the anti-epileptic effect of Pingxian granules.Method： Totally 60 45-days-old Wistar rats were selected and then randomly assigned into 5 groups： the normal control group,the model group,the positive control group,the Pingxian high dose group and the Pingxian low dose group,with 12 in each group.Except the normal control group,all the groups were intraperitoneally injected with 35 mg·kg^-1 pentylenetetrazol to establish the models of epilepsy.The Pingxian high dose（1.66 g·mL^-1） and low dose（0.42 g·mL^-1） groups were intragastrically infused with Pingxian granules 2 mL·d^-1.The positive control group received 3.6 g·L^-1 phenobarbital suspension by gastric perfusion.The normal group and the model group were drenched with distilled water,2 mL·d^-1,for 5 weeks.Bcl-2 and Bax protein positive cells were labeled with immunohistochemical SABC at 3,5 w.Result： ① Rats in the model group appeared the epileptic behavior at the 1st week,and became serious with the kindle frequency;grade Ⅵepileptic behavior appeared at the 4th week.The attack frequency and grade of the Pingxian group were less and lower,the highest grade were only Ⅳ,and there were no significant differences in the attack grade and frequency.②With the increase in kindle frequency,the model group showed a notable decrease in the Bcl-2 expression compared with the normal control group at the 3rd and 5th weekend（P〈0.01）,but a significant increase in Bax protein expression（P〈0.01）.The number of the Bcl-2 protein expression in Pingxian groups and the positive control group were obviously more than the model group（P〈0.01）;and the number of the Bax protein expression in Pingxian groups and the positive control group were obviously less than the model group（P〈0.01）.Conclusion： Pingxian granules may decrease neuronal cell apoptosis by improving the protein expression of apoptosis regulatory gene Bcl-2 and inhibiting the protein expression of apoptosis regulatory gene Bax with a view of anti-epilepsy.