文献类型：期刊文献

英文题名：Polysaccharides from Dicliptera chinensis ameliorate liver disturbance by regulating TLR-4/NF-κB and AMPK/Nrf2 signalling pathways

作者：Zhang Kefeng[1,2];Xu Qiongmei[1];Gao Ya[1];Cao Houkang[2];Lian Yuanyu[1];Li Zimeng[1];Xu Jie[1];Zhong Mingli[1];Li Jiani[1];Wei Riming[1];Dong Jianghui[1];Jin Ling[2]

第一作者：Zhang Kefeng

通信作者：Dong, JH[1];Jin, L[2]

机构：[1]Guilin Med Univ, Coll Pharm, Guilin 541199, Guangxi, Peoples R China;[2]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Gansu, Peoples R China

第一机构：Guilin Med Univ, Coll Pharm, Guilin 541199, Guangxi, Peoples R China

通信机构：[1]corresponding author), Guilin Med Univ, Coll Pharm, Guilin 541199, Guangxi, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Gansu, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2020

卷号：24

期号：11

起止页码：6397

外文期刊名：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000528482300001)】；

基金：National Natural Science Foundation of China, Grant/Award Number: 81960779, 81760114, 81660104 and 81860673; National Science Foundation of Guangxi Province of China, Grant/Award Number: 2017GXNSFAA198218, 2017GXNSFAA198326 and 2018GXNSFAA281040

语种：英文

外文关键词：AMPK/Nrf2; Dicliptera chinensis polysaccharides; liver disturbances; TLR-4/NF-kappa B

摘要：The purpose of this study was to alleviate liver disturbance by applying polysaccharides from Dicliptera chinensis (DCP) to act on the adenosine monophosphate-activated protein kinase/ nuclear factor erythroid 2-related factor 2 (AMPK/ Nrf2) oxidative stress pathway and the Toll-like receptor 4 (TLR-4)/ nuclear factor kappa-B (NF-kappa B) inflammatory pathway and to establish an in vivo liver disturbance model using male C57BL/6J and TLR-4 knockout ((-/-)) mice. For this, we evaluated the expression levels of SREBP-1 and Nrf2 after silencing the expression of AMPK using siRNA technology. Our results show that with regard to the TLR-4/ NF-kappa B inflammatory pathway, DCP inhibits TLR-4, up-regulates the expression of peroxisome proliferator-activated receptor-gamma (PPAR-gamma), reduces the expression of phospho(p)-NF-kappa B and leads to the reduction of downstream inflammatory factors, such as tumour necrosis factor-alpha (TNF-alpha), interleukin (IL)-6 and IL-1 beta, thereby inhibiting the inflammatory response. Regarding the AMPK/ Nrf2 oxidative stress pathway, DCP up-regulates the expression of p-AMPK and Nrf2, in addition to regulating glucose and lipid metabolism, oxidative stress and ameliorating liver disturbance symptoms. In summary, our study shows that DCP alleviates liver disturbances by inhibiting mechanisms used during liver inflammation and oxidative stress depression, which provides a new strategy for the clinical treatment of liver disturbance.