文献类型：期刊文献

中文题名：当归有效组分防治阿尔茨海默病模型大鼠组方配伍优化的实验研究

英文题名：Compatibility optimization of composing prescription on active components of Danggui (Radix Angelicae senensis) for prevention and cure of Alzheimer’s disease (AD)

作者：王虎平[1,2];吴红彦[1,2];李海龙[2,3];马春林[1];曾庆涛[1];崔淑梅[1];朱凯敏[1]

第一作者：王虎平

机构：[1]甘肃中医药大学基础医学院,甘肃兰州730000;[2]甘肃省方药挖掘与创新转化重点实验室,甘肃兰州730000;[3]甘肃中医药大学临床医学院,甘肃兰州730000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2019

卷号：0

期号：3

起止页码：12

中文期刊名：甘肃中医药大学学报

外文期刊名：Journal of Gansu University of Chinese Medicine

基金：甘肃省自然科学基金项目(1212RJZA075);甘肃省中医方药挖掘与创新转化重点实验室; 甘肃省中药新产品创制工程实验室开放基金项目(ZYFYZH-KJ-2016-001)

语种：中文

中文关键词：阿尔茨海默病;当归;有效组分;当归多糖;当归挥发油;阿魏酸;Morris水迷宫;配伍优化

外文关键词：Alzheimer’s disease(AD);Danggui(Radix Angelicae senensis);active components;Angelicae senensis polysaccharide;Angelicae senensis volatile oil;ferulic acid;Morris water maze;compatibility optimization

摘要：目的优化当归有效组分当归多糖、当归挥发油及阿魏酸防治阿尔茨海默病(AD)的最佳配伍。方法从150只SPF级Wistar大鼠中随机选取10只作为空白组、10只作为假手术组,其余大鼠均在脑立体定位下于海马CA1区左右两侧分别注射1μL Aβ25-35复制AD大鼠模型,筛选造模成功的110只大鼠,随机分为模型组、当归组及当归组分配伍1~9组等11组,每组10只。造模第7天开始灌胃给药,空白组、假手术组与模型组均灌胃生理盐水,当归组灌胃当归水煎液,当归组分配伍各组灌胃对应药液,连续灌胃28 d。给药第24~27天进行Morris水迷宫定位航行试验,第28天进行空间探索试验。当归组分配伍组以当归多糖、当归挥发油、阿魏酸为考察因素,采用正交试验法,以各组大鼠逃避潜伏期和跨原平台次数为考察指标,优选当归有效组分防治AD的最佳配伍。结果与假手术组比较,模型组大鼠逃避潜伏期明显延长,跨原平台次数明显减少,差异均有统计学意义(P<0.05或P<0.01)。与模型组比较,各给药组大鼠逃避潜伏期均不同程度缩短,差异有统计学意义(P<0.05或P<0.01);当归组,当归组分配伍1,2,5,6,7,9组跨原平台次数明显增多,差异均有统计学意义(P<0.05或P<0.01)。与当归组比较,当归组分配伍1,4,5,6,7,8组逃避潜伏期明显缩短,差异均有统计学意义(P<0.05或P<0.01)。当归有效组分改善AD模型大鼠学习记忆能力的最佳配伍是1 m L组分配伍药液中含当归多糖0.017 6 g、当归挥发油0.004 4 m L、阿魏酸0.001 2 g。结论当归有效组分配伍能够有效地改善AD模型大鼠的学习记忆能力,对AD具有一定的防治作用。
Objective To optimize the best compatibility between Angelicae senensis polysaccharide and volatile oil as well as ferulic acid of the active components in Danggui(Radix Angelicae senensis)for the prevention and cure of Alzheimer’s disease(AD).Methods Of 150 SPF Wistar rats,10 were randomly chosen as blank group,another 10 were chosen as sham operation group,and the rest of them were injected 1μL Aβ25-35 into the left and right sides of the CA1 subfield of the hippocampal formation with stereotaxic apparatus to replicate AD rats model,and 110 successful rats model were screened and randomly divided into 11 groups,including model group,Danggui group and 1 to 9 Danggui component compatibility groups,with 10 rats in each.The rats in the blank group,shamoperation group and model group were given normal saline by intragastric administration on the 7 th day of modeling,the rats in the Danggui group were given Danggui water decoction by intragastric administration,and the rats in the Danggui component compatibility groups were given corresponding soup by intragastric administration for 28 consecutive days.The Morris water maze place navigation test was conducted on the 24 th to 27 th day of drug administration,and the spatial probe test was conducted on the 28 th day.In the Danggui component compatibility groups,the orthogonal experiment was adopted with polysaccharide,volatile oil and ferulic acid in Danggui as investigation factors,and with the rats’escape latency and the times of leaping over platform as investigation index to optimize the best compatibility of active components in Danggui for prevention and cure of AD.Results Compared with sham operation group,the escape latency significantly prolonged and the times of leaping over platform significantly decreased of rats in the model group,and the difference was statistically significant(P<0.05 or P<0.01).Compared with the model group,the escape latency significantly shortened in rats of each medication administration groups,and the difference was statistically significant(P<0.05 or P<0.01).In Danggui group and the 1st,2nd,5th,6th,7th and 9th Danggui component compatibility groups,the times of leaping over platform significantly increased,and the difference was statistically significant(P<0.05 or P<0.01).Compared with Danggui group,the escape latency significantly shortened in the 1st,4th,5th,6th,7th and 8th Danggui component compatibility groups,and the difference was statistically significant(P<0.05 or P<0.01).The best compatibility of active components of Danggui in improving learning and memory ability of AD rats model is 1 m L compatible soup containing Angelicae senensis polysaccharide 0.017 6 g and volatile oil 0.004 4 m L and ferulic acid 0.001 2 g.Conclusion The compatibility of active components of Radix Angelicae sinensis can effectively improve the learning and memory ability of AD rats model,and has certain prevention and cure effect on AD.