文献类型：期刊文献

中文题名：基于全转录组测序技术的单眼视觉剥夺大鼠针刺响应基因的钓取及功能鉴定

英文题名：Screening and biological function analysis of acupuncture response genes based on RNA-seq in monocular vision-deprived rats

作者：阚丽丽[1];刘安国[2];马重兵[2];王觉[2];孙燕[2];朱田田[2];严兴科[2]

第一作者：阚丽丽

机构：[1]甘肃省中医院,兰州730050;[2]甘肃中医药大学针灸推拿学院,兰州730000

第一机构：甘肃省中医院,兰州730050

年份：2021

卷号：36

期号：11

起止页码：6674

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金项目(No.81660816,No.81860879,No.81260560);甘肃省高等学校创新能力项目(No.2019A-073);中医药传承创新平台建设项目。

语种：中文

中文关键词：“调气通经明目”针法;单眼视觉剥夺;全转录组测序;针刺响应基因;视觉可塑性机制

外文关键词：‘Tiaoqi-Tongjing-Mingmu’acupuncture therapy;Monocular visual deprivation;RNA-seq;Acupuncture response gene;Mechanism of visual plasticity

摘要：目的:基于全转录组测序(RNA-seq)技术揭示"调气通经明目"针法干预调节视觉剥夺效应的分子生物学机制。方法:将72只SD幼鼠随机分为空白组、空白捆绑组、空白针刺组、模型组、模型捆绑组及模型针刺组,每组12只。对模型组、模型捆绑组和模型针刺组幼鼠行右眼视觉剥夺模型复制。空白针刺组、模型针刺组于造模第3天开始针刺治疗,选取睛明、风池、光明、攒竹4穴,每日针刺1次,留针10min,共治疗14d,各穴双侧隔天交替使用。模型捆绑组和空白捆绑组给予每天相同时间的捆绑刺激。14d后对各组大鼠行视传导通路图形视觉诱发电位(P-VEP)检测,对视皮质区组织行RNA-Seq测序,钓取特异性针刺响应基因,并对其生物学功能进行预测。结果:与空白组比较,模型组、模型捆绑组P;波潜时显著延长(P<0.05),振幅显著缩短(P<0.05);与模型组比较,模型针刺组P;波潜时显著缩短(P<0.05),振幅延长(P<0.05)。通过差异基因分析,共钓取左侧视皮质特异性针刺响应基因38个,涉及11个GO条目;钓取右侧视皮质特异性针刺响应基因63个,涉及29个GO条目。结论:"调气通经明目"针法可引起单眼视觉剥夺大鼠视皮质特异性响应基因的表达,并从转录、翻译层面有效干预和调节视觉剥夺性损害。
Objective: To discuss the biological mechanism of ‘Tiaoqi-Tongjing-Mingmu’ acupuncture intervention on visual deprivation by RNA-seq technique. Methods: A total of 72 14-day-old SD rats were divided into blank group,blank binding group, blank acupuncture group, model group, model binding group and acupuncture group, 12 rats in each group. The rats in model group, model binding group and acupuncture group were established deprivation amblyopia model.Rats in blank acupuncture group and acupuncture group were treated with acupuncture from the third day after the model was established. Jingming(BL1),Fengchi(GB20), Cuanzhu(BL2), Guangming(GB37) were selected for acupuncture once a day and for a total of 14 days. The bilateral acupoints were used alternately every day. The binding stimulation time of model binding group and blank binding group were the same every day. After 14 days, P-VEP was detected on visual conduction pathway of each group, and RNA-seq was used for the visual cortex gene express. Then the specific response gene of acupuncture was screened and its biological function was predicted. Results: Compared with the blank group, the latent time of P;wave in the model group and the model binding group was significantly longer(P<0.05), and the amplitude was shorter(P<0.05). Compared with model group, the latent time of P;wave in acupuncture group were shorter(P<0.05), and amplitude was lorger(P<0.05). A total of 38 response genes of left visual cortex and 63 response genes of right visual cortex were retrieved. There were 11 and 29 GO items in left and right visual cortex differential genes, respectively. Conclusion:‘Tiaoqi-Tongjing-Mingmu’ acupuncture treatment in the sensitive period of monocular visual deprivation rats can cause the expression and inhibition of specific acupuncture response genes in the visual cortex. These genes are effectively intervened from the transcription and translation level. Regulate visual deprivation damage and promote the recovery of visual function.