文献类型：期刊文献

中文题名：丹参酮ⅡA联合伊马替尼对慢性髓细胞白血病K562细胞作用的实验研究

英文题名：Effect of Tanshinone ⅡA Combined with Imatinib on K562 Cells of Chronic Myelocytic Leukemia

作者：李丹丹[1];张金花[1];王秀月[1];陈彻[1];王晶[1]

第一作者：李丹丹

机构：[1]甘肃中医药大学临床医学院,甘肃兰州730000

第一机构：甘肃中医药大学临床医学院

年份：2017

卷号：45

期号：5

起止页码：57

中文期刊名：中医药学报

外文期刊名：Acta Chinese Medicine and Pharmacology

收录：CSTPCD

基金：甘肃省高等学校基本科研业务费专项资金(2013-005);甘肃省中药药理与毒理学重点实验室开放基金项目(ZDSYS-KJ-2016-002)

语种：中文

中文关键词：丹参酮ⅡA;伊马替尼;K562细胞;凋亡;线粒体

外文关键词：Tanshinone Ⅱ ; Imatinib ; K562ceUs ; Apoptosis ; Mitochondria

摘要：目的:探讨丹参酮ⅡA联合伊马替尼对K562细胞凋亡作用的影响及相关作用机制。方法:将K562细胞分为6组:常规培养K562细胞组、1μM伊马替尼单药组、20μM丹参酮ⅡA单药组,以及5μM、10μM、20μM丹参酮ⅡA分别与1μM伊马替尼的联合用药组。在显微镜下观察细胞的形态学变化,检测细胞12 h和24 h的生长情况,利用流式细胞仪检测细胞的凋亡情况,再利用JC-1染料在荧光显微镜下观察细胞线粒体膜电位变化情况。结果:丹参酮ⅡA通过抑制线粒体膜电位,促进细胞凋亡(P<0.05);并随丹参酮ⅡA的浓度增加联合用药组的抑制作用也增强。结论:丹参酮ⅡA可显著增强伊马替尼引起的K562细胞凋亡,其机制与线粒体途径有关。
Objective ： To investigate the effect of Tanshinone ⅡA with Imatinib on the apoptosis of K562 ceils and its re- lated mechanisms. Methods： K562 cells were cultured and divided into six groups： normal K562 cells group, 20 μM tanshinone ⅡA group, 1 μM Imatinib group, and combined group（5μM, 10 μM, and 20 μM respectively plus 1μM Imatinib）. After treatment, the morphological changes of cells were observed under microscope; the cell apoptosis was detected by flow cytometry; and the mitochondrial activity was also observed by using JC - 1 reagent and fluorescence microscope. Results： Tanshinone ⅡA promotes the cell apoptosis by inhibiting the mitochondrial transmembrane poten- tial （ P 〈 0.05 ） ; and the inhibitory effects of tanshinone ⅡA were enhanced with the increase of its concentration. Con- clusion ： Tanshinone ⅡA can significantly enhance the apoptosis of K562 treated by Imatinib, which is related with mito- chondrial pathway.