文献类型：期刊文献

中文题名：纹党连作土壤浸提液对其种子萌发及幼苗生长的自毒效应研究

英文题名：A study of autotoxic effects of soil extract from continuously cropped Codonopsis pilosula var.modesta on seed germination and seedling growth

作者：陈莺宇[1];邱亚娟[1];邵晓龄[1];黄钰芳[1,2,3];杨扶德[1];陈林杰[1];陈红刚[1,2];谢田朋[1,2]

第一作者：陈莺宇

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]西北中藏药省部共建协同创新中心,甘肃兰州730000;[3]甘肃中医药大学,敦煌医学与转化教育部重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2024

卷号：33

期号：12

起止页码：122

中文期刊名：草业学报

外文期刊名：Acta Prataculturae Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金(82160714);甘肃省自然科学基金(21JR1RA271);国家重点研发计划(2018YFC1706305);甘肃省高等学校产业支撑引导项目(2021CYZC-40);研究生“创新之星”项目(2023CXZX-764);敦煌医学与转化教育部重点实验室项目(DHYX20-13)资助。

语种：中文

中文关键词：纹党;连作障碍;自毒作用;气质联用技术;自毒物质

外文关键词：Codonopsis pilosula var.modesta;continuous cropping obstacles;autotoxicity;GC-MS;autotoxic substance

摘要：为探究纹党连作土壤浸提液对其种子萌发及幼苗生长的自毒效应,并筛选出其中潜在的化感自毒物质,选取连作1、2和3年的纹党根际土壤,利用种子发芽试验及幼苗生长试验研究其浸提液对纹党种子萌发和幼苗生长的影响,并利用气质联用技术(GC-MS)对根际土壤中的自毒物质进行鉴定。结果表明,各连作年限土壤浸提液对纹党种子的萌发及幼苗的生长均表现出“低促高抑”的影响趋势;在同等抑制浓度条件下,连作3年的抑制作用最强,连作1年的最弱。纹党幼苗叶片叶绿素的含量以及过氧化氢酶、过氧化物酶、超氧化物歧化酶的活性随土壤浸提液浓度的增大先升高后降低,丙二醛、脯氨酸的含量随土壤浸提液浓度的增大而上升,且连作3年的影响效应最强。GCMS结果显示,连作1、2和3年土壤浸提液中分别检测出12、18和8种化学物质,包括烃类、醇类、酮类及脂肪酸类等多种化合物。纹党连作土壤浸提液对其种子萌发和幼苗生长具有自毒效应;自毒作用是纹党连作障碍发生的原因之一。
Autotoxicity is one of the serious problems in Codonopsis pilosula var.modesta cultivation.The purpose of this study was to investigate the autotoxicity effects of soil extract from continuously cropped C.pilosula var.modesta on seed germination and seedling growth,and to screen out potential autotoxic compounds of C.pilosula var.modesta.Rhizosphere soil of C.pilosula var.modesta continuously cropped for 1,2 and 3 years was collected;extracts from the collected soil were prepared,and germination and seedling growth tests for C.pilosula var.modesta were conducted.Potentially autotoxic compounds in the rhizosphere soil extracts were identified by gas chromatography-mass spectrometry(GC-MS).It was found that the soil extract for all three continuous cropping durations had an effect of“low promotion and high inhibition”on the seed germination and seedling growth of C.pilosula var.modesta.However,the inhibition effect of continuous cropping for 3 years was the strongest,and that of continuous cropping for 1 year was the weakest.The chlorophyll content and the catalase,peroxidase and superoxide dismutase in leaves initially increased and then decreased with increase in soil extract concentration,and the contents of malondialdehyde and proline increased with increase in soil extract concentration.Again,these effects were strongest for extracts from soils after three years of continuous cropping.The GC-MS analysis detected,respectively,12,18 and 8 different potentially autotoxic compounds(including hydrocarbons,alcohols,ketones and fatty acids),in extracts from soils continuously cropped for 1,2 and 3 years.In summary,extracts from soils continuously cropped with C.pilosula var.modesta had autotoxic effects on seed germination and seedling growth.Autotoxicity is one of the causes of continuous cropping vigor loss in C.pilosula var.modesta.