文献类型：期刊文献

英文题名：Impact of gut microbiota on atypical endometrial hyperplasia and endometrial cancer: a comprehensive analysis of microbial composition and metabolomic profiling

作者：Peng, Haochen[1];Chen, Jiayu[2];Shao, Yawen[1];Li, Juan[1];Chen, Mali[1];He, Chunxiao[1];Sun, Jianhao[1];Wang, Weihong[1];Cao, Xiaocui[1];Yang, Hong[1];Zhou, Jia[1];Wu, Zhenzhen[1,2]

第一作者：Peng, Haochen

通信作者：Wu, ZZ[1];Wu, ZZ[2]

机构：[1]Gansu Prov Cent Hosp, Gansu Prov Matern & Child Care Hosp, Lanzhou, Peoples R China;[2]Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou, Peoples R China

第一机构：Gansu Prov Cent Hosp, Gansu Prov Matern & Child Care Hosp, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Gansu Prov Cent Hosp, Gansu Prov Matern & Child Care Hosp, Lanzhou, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou, Peoples R China.|[10735]甘肃中医药大学;

年份：2026

卷号：26

期号：1

外文期刊名：BMC MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001693244000001)】；

基金：This work was supported by grants from the Natural Science Foundation of Gansu Province (22JR5RA718 and 24JRRA621).

语种：英文

外文关键词：Gut microbiota; Endometrial cancer; Lipid metabolism; Biomarkers; Metabolomics

摘要：Background Endometrial cancer (EC) is one of the most common malignant tumors in women, and in recent years, the role of gut microbiota in tumorigenesis has gradually gained attention. Previous studies have shown that the gut microbiome is closely related to the occurrence of various cancers, but the specific mechanisms through which gut microbiota contribute to the development of endometrial cancer (EC) remain unclear. This study aims to analyze the gut microbione characteristics of atypical endometrial hyperplasia (AEH) and EC, and to explore key gut microbial species and metabolites, providing evidence for the etiological research and early screening of EC and AEH.
Methods This study selected 24 AEH or EC patients from the Gynecology Department of Gansu Provincial Maternity and Child Care Hospital between February 2023 and October 2023. The patients were divided into the AEH group (n = 7) and the EC group (n = 17) , with 24 healthy women selected as a control group. Fecal and serum samples were collected, and 16S rRNA gene sequencing was performed using the Illumina MiSeq platform. Serum metabolomics analysis was conducted using LC-MS technology. Spearman correlation analysis was used to explore the associations between gut microbiota and metabolites, and potential gut microbial biomarkers were evaluated using ROC analysis.
Results The study found that as AEH progressed to EC, significant changes occurred in the composition of the gut microbiota, particularly in Klebsiella, whose abundance increased from 0.264% in the control group to 0.809% in the AEH group and 6.092% in the EC group, with significant differences (P<0.001, FDR-0.026). LEfSe analysis identified Megamonas, Klebsiella, Escherichia, and Akkermansia as potential biomarkers. ROC analysis showed that the AUCs of Megamonas/Klebsiella for EC were 0.864/0.838, and the AUCs of Escherichia/Akkermansia for AEH were 0.744/0.920. Metabolomics analysis revealed significant enrichment of glycerophospholipid metabolism in both the EC and AEH groups, with significant differences in lipid metabolism in the EC group. Correlation analysis indicated significant positive correlations between Enterococcus and hypoxanthine, inosine in the EC group (r=0.686, 0.637, P<0.05). Conclusion This study reveals the dynamic changes in the gut microbiome during the development of endometrial lesions, especially the increasing abundance of Klebsiella as AEH progresses to EC, suggesting that it may play a key role in the occurrence and progression of EC. Furthermore, significant changes in lipid metabolism further support the role of gut microbiota in regulating lipid metabolism in EC pathogenesis. This study provides new insights into the role of gut microbiota in endometrial cancer and offers a theoretical basis for early diagnosis and personalized treatment strategies based on gut microbiota.