文献类型：期刊文献

中文题名：蛇葡萄素钠协同卡铂抑制人肺腺癌SPC-A-1细胞增殖

英文题名：Cytotoxic effect of ampelopsin sodium combined with carboplatin on human lung adenocarcinoma cell line SPC-A-1

作者：徐明丽[1,2];韩伟[3];吴勇杰[1]

第一作者：徐明丽

机构：[1]兰州大学基础医学院药理学研究所甘肃省新药临床前研究重点实验室;[2]甘肃省卫生学校;[3]甘肃省定西市卫生学校

第一机构：兰州大学基础医学院药理学研究所甘肃省新药临床前研究重点实验室

年份：2011

卷号：16

期号：8

起止页码：890

中文期刊名：中国临床药理学与治疗学

外文期刊名：Chinese Journal of Clinical Pharmacology and Therapeutics

收录：CSTPCD;;CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：蛇葡萄素钠;卡铂;人肺腺癌SPC-A-1细胞;细胞凋亡

外文关键词：Ampelopsin sodium; Carboplatin; Human lung adenocarcionma cell line SPC-A-1; Apoptosis

摘要：目的:探讨蛇葡萄素钠(AMP-Na)单用及其与卡铂(CBP)合用对SPC-A-1人肺腺癌细胞增殖的抑制作用及可能的机制。方法:采用MTT比色法测定AMP-Na单用及其与CBP合用对SPC-A-1细胞的体外细胞毒活性;用流式细胞仪分析AMP-Na单用及与卡铂合用后对SPC-A-1细胞凋亡的影响。结果:体外细胞毒实验和流式细胞仪结果表明,AMP-Na对人肺腺癌SPC-A-1细胞增殖的抑制作用具有明显的量效关系,与CBP联用后凋亡率较卡铂单用明显上升,有协同抑制作用。结论:AMP-Na单用能够显著抑制人肺腺癌SPC-A-1细胞的增殖,与卡铂合用可以增强卡铂的活性,协同抑制SPC-A-1细胞的增殖;AMP-Na可能是通过诱导凋亡而发挥其抗肿瘤作用。
AIM： To investigate the cytotoxic effect and mechanism of ampelopsin sodium（AMP-Na） on human lung adenocarcionma cell line SPC-A-1 by alone or combined with carboplatin. METHODS： The inhibitory effects on proliferation of human lung cancer cell line SPC-A-1 were examined by MTT colorimetric assay.Flow cytometry（FCM） was employed to analyze the cell apoptosis.RESULTS：Experimental study on cytotoxicity in vitro showed that AMP-Na decreased the survival rate of SPC-A-1 cells in a concentration-dependent manner.There was a synergistic cytotoxic effect on SPC-A-1 cells treated with AMP-Na combined with carboplatin.The apoptosis rate in group treated with AMP-Na combined with carboplatin was significantly increased as compared with that in group treated with carboplatin alone.CONCLUSION： AMP-Na decreases SPC-A-1 cells survival rate in a concentration dependent manner,and shows significant cytotoxic activity.There is a synergistic cytotoxic effect on SPC-A-1 cells treated with AMP-Na combined with carboplatin.The mechanism of AMP-Na is probably that it can induce SPC-A-1 cells apoptosis.