文献类型：期刊文献

中文题名：微波消解-离子色谱法测定枸杞多糖的含量及组成

英文题名：Determination of the content and composition of Lycium barbarum polysaccharides by microwave digestion-ion chromatography

作者：颉东妹[1,2,3];王宁丽[2];刘笑笑[3];吴福祥[3];裴栋[2];郭玫[1];邸多隆[2]

第一作者：颉东妹

机构：[1]甘肃中医药大学,药学院,兰州730000;[2]中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室,兰州730000;[3]兰州市食品药品检验检测研究院,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2022

卷号：13

期号：4

起止页码：1065

中文期刊名：食品安全质量检测学报

外文期刊名：Journal of Food Safety and Quality

收录：CSTPCD;;北大核心:【北大核心2020】；

基金：甘肃省重点研发计划项目(2021-0202-NCC-0163);甘肃省青年科技基金计划项目(20JR10RA054);中国科学院“西部之光”人才培养引进计划-西部青年学者项目(A类);甘肃省科技计划项目(21YF5NA135、21CX6NF234)。

语种：中文

中文关键词：微波辅助消解;枸杞多糖;离子色谱法

外文关键词：microwave-assisted digestion;Lycium barbarum polysaccharides;ion chromatography

摘要：目的 建立微波消解-离子色谱法测定枸杞多糖含量及组成的方法。方法 分别考察了三氟乙酸浓度、微波消解温度及微波消解时间对枸杞多糖消解效率的影响,优化了离子色谱法检测枸杞多糖的色谱条件,并对所建立的方法进行方法学评价。结果 枸杞多糖最佳消解条件为温度120℃、时间20 min、三氟乙酸浓度3 mol/L;经方法学评价, 9种单糖和2种糖醛酸均具有良好的线性关系,相关系数均大于0.98;精密度相对标准偏差小于3.00%,检出限和定量限良好。通过对枸杞中单糖组成的分析,表明在枸杞中葡萄糖、木糖、半乳糖、甘露糖、阿拉伯糖、鼠李糖、果糖、岩藻糖、核糖、半乳糖醛酸、葡萄糖醛酸是组成枸杞多糖的主要成分。结论 微波辅助消解方法可以大大缩减枸杞多糖的消解时间,可用于快速检测枸杞多糖的含量。
Objective To establish a method for the determination of the content and composition of Lycium barbarum polysaccharides by microwave digestion-ion chromatography. Methods A single factor experiment was used to investigate the effects of trifluoroacetic acid concentration, microwave digestion temperature and microwave digestion time on the hydrolysis efficiency of Lycium barbarum polysaccharides, respectively. The chromatographic conditions for the determination of Lycium barbarum polysaccharide by ion chromatography conditions were optimized, and the method was evaluated. Results The optimal hydrolysis conditions for Lycium barbarum polysaccharides were that digestion temperature was 120 ℃, digestion time was 20 min, and trifluoroacetic acid concentration was 3 mol/L. After methodological evaluation, 9 kinds of monosaccharides and 2 kinds of uronic acids had good linear relationship, the correlation coefficients were all greater than 0.98;the relative standard deviations of precision were less than 3.00%, and the limits of detection and quantification were good. The analysis of monosaccharides in Lycium barbarum showed that glucose, xylose, galactose, mannose, arabinose, rhamnose,fructose, fucose, ribose, galacturonic acid and glucuronic acid were the main components of Lycium barbarum polysaccharide.Conclusion The microwave-assisted digestion method can greatly reduce the digestion time of Lycium barbarum polysaccharide, and can be used to rapidly detect the content of Lycium barbarum polysaccharide.