文献类型：期刊文献

中文题名：当归挥发油联合卡铂对人肺腺癌A549细胞增殖及凋亡的影响

英文题名：Effect of volatile oil of the angelica combined with carboplatin on the proliferation and apoptosis of human lung adenocarcinoma cell A549

作者：张艳霞[1];张正顺[2];陈绍仪[3];孔学礼[1];周佳玮[1]

第一作者：张艳霞

机构：[1]甘肃中医药大学/甘肃省高校中(藏)药化学与质量研究省级重点实验室;[2]甘肃省中医院白银分院;[3]白银市第二人民医院

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2017

卷号：22

期号：7

起止页码：755

中文期刊名：中国临床药理学与治疗学

外文期刊名：Chinese Journal of Clinical Pharmacology and Therapeutics

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：白银市科技计划项目(2013-2-76Y);甘肃中医学院中青年科研基金项目(2305016601)

语种：中文

中文关键词：当归挥发油;卡铂;人肺腺癌A549细胞;细胞凋亡

外文关键词：volatile oil of the angelica; carboplatin; human lung adenocarcinoma cell A549; apoptosis

摘要：目的:探讨当归挥发油与卡铂联用对人肺腺癌A549细胞增殖及凋亡的影响。方法:MTT比色法检测两药单用及联用对A549细胞的体外杀伤活性;用流式细胞术检测A549细胞凋亡;AO/BE双荧光染色法观察A549细胞凋亡。结果:当归挥发油单独应用及与卡铂合用对A549细胞的增殖均具有浓度依赖性抑制作用,两药联用IC_(50)较单用IC_(50)下降,说明当归挥发油对卡铂抑制A549细胞增殖的作用具协同效应。流式细胞术检测结果表明,当归挥发油单用及与卡铂合用均可诱导A549细胞凋亡、坏死,两药合用后细胞凋亡、坏死率显著升高。AO/BE双荧光染色法结果显示,联合用药组细胞凋亡、坏死率上升。结论:当归挥发油与卡铂具有协同诱导A549细胞凋亡的作用。
AIM： To investigate the cytotoxic effect and apoptosis of volatile oil of the angelica on human lung adenocarcinoma cell A549 combined with carboplatin. METHODS： Use the MTT colorimetric method to investigate cytotoxicity effect of volatile oil of the angelica alone or combined with carboplatin to human lung adenocarcionma cell A549; Use flow cytometry （FCM）analysis the cell apoptosis. Observe the cell apoptosis by AO/BE fluorescent staining method. RESULTS： The A549 cell was inhibited by volatile oil of the angelica alone or combined with carboplatin in a concentration-dependent manner, and compared with use volatile oil of the angelica alone, the IC50 descend in drug combination group. Results of flow cytometry indicates that, volatile oil of the angelica alone or combined with carboplatin, could induce apoptosis and necro-sis of A549 cell, and compared with use volatile oil of the angelica alone, the rate of apoptosis and necrosis all rise in drug combination group. The results of AO/BE fluorescent staining method displayed that, the rate of apoptosis and necrosis were all raised in drug combination group. CONCLUSION： Volatile oil of the angelica had depressant effect on the generation of A549 cell, there is a synergistic cytotoxic effect on A549 cells treated with volatile oil of the angelica combined with carboplatin. The mechanism is probably that it can induce apoptosis of A549 cell.